BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15200

Title: NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster   PubMed: 19636833

Authors: Pauleta, Sofia; Moura, Jose

Citation: Pauleta, Sofia; Duarte, Americo; Carepo, Marta; Pereira, Alice; Tavares, Pedro; Moura, Isabel; Moura, Jose. "NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster"  Biomol. NMR Assignments 1, 81-83 (2007).

Assembly members:
ApoORP, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Desulfovibrio gigas   Taxonomy ID: 879   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio gigas

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ApoORP: ASHMQRIAVTAEGPGLDGLV DPRFGRAAGFVVVDAATMAA EYVDNGASQTLSHGAGINAA QVLAKSGAGVLLTGYVGPKA FQALQAAGIKVGQDLEGLTV RQAVQRFLDGQVPMAAGPNK

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts523
15N chemical shifts135
1H chemical shifts773

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 120 residues - Formula weight is not available

1   ALASERHISMETGLNARGILEALAVALTHR
2   ALAGLUGLYPROGLYLEUASPGLYLEUVAL
3   ASPPROARGPHEGLYARGALAALAGLYPHE
4   VALVALVALASPALAALATHRMETALAALA
5   GLUTYRVALASPASNGLYALASERGLNTHR
6   LEUSERHISGLYALAGLYILEASNALAALA
7   GLNVALLEUALALYSSERGLYALAGLYVAL
8   LEULEUTHRGLYTYRVALGLYPROLYSALA
9   PHEGLNALALEUGLNALAALAGLYILELYS
10   VALGLYGLNASPLEUGLUGLYLEUTHRVAL
11   ARGGLNALAVALGLNARGPHELEUASPGLY
12   GLNVALPROMETALAALAGLYPROASNLYS

Samples:

sample_1: ApoORP, [U-15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 1 mM; D2O 10%

sample_2: ApoORP, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 1 mM; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAI59607
GB AGW13746
REF WP_021760641