BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15198

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for all-Ala-HEWL   PubMed: 16258828

Authors: Schloerb, Christian; Ackermann, Katrin; Richter, Christian; Schwalbe, Harald

Citation: Schloerb, Christian; Ackermann, Katrin; Richter, Christian; Wirmer, Julia; Schwalbe, Harald. "Heterologous expression of hen egg white lysozyme and resonance assignment of tryptophan side chains in its non-native states"  J. Biomol. NMR 33, 95-104 (2005).

Assembly members:
all-Ala-Hen_egg_white_lysoyzme, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
all-Ala-Hen_egg_white_lysoyzme: MKVFGRAELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWANDGRTPGSRNLANIP ASALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGARL

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts125
1H chemical shifts427

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1all-Ala-hen egg white lysozyme1

Entities:

Entity 1, all-Ala-hen egg white lysozyme 130 residues - Formula weight is not available

1   METLYSVALPHEGLYARGALAGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   ALAALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPALAASNASPGLYARGTHR
8   PROGLYSERARGASNLEUALAASNILEPRO
9   ALASERALALEULEUSERSERASPILETHR
10   ALASERVALASNALAALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGALALYSGLYTHRASP
13   VALGLNALATRPILEARGGLYALAARGLEU

Samples:

sample_1: all-Ala-Hen egg white lysoyzme, [U-99% 13C; U-99% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 2.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

CARA v1.5, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 11051 11052 11459 11460 11461 11462 18365 18366 18367 18368 18369 18370