BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15197

Title: 1H, 15N, and 13C chemical shift assignments for CaBP1   PubMed: 19636832

Authors: Ames, James

Citation: Li, Congmin; Ames, James. "1H, 15N, and 13C chemical shift assignments of calcium-binding protein 1 (CaBP1)"  Biomol. NMR Assignments 1, 77-79 (2007).

Assembly members:
CaBP1, polymer, 167 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CaBP1: MGNCVKYPLRNLSRKDRSLR PEEIEELREAFREFDKDKDG YINCRDLGNCMRTMGYMPTE MELIELSQQINMNLGGHVDF DDFVELMGPKLLAETADMIG VKELRDAFREFDTNGDGEIS TSELREAMRKLLGHQVGHRD IEEIIRDVDLNGDGRVDFEE FVRMMSR

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts593
15N chemical shifts145
1H chemical shifts839

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 167 residues - Formula weight is not available

1   METGLYASNCYSVALLYSTYRPROLEUARG
2   ASNLEUSERARGLYSASPARGSERLEUARG
3   PROGLUGLUILEGLUGLULEUARGGLUALA
4   PHEARGGLUPHEASPLYSASPLYSASPGLY
5   TYRILEASNCYSARGASPLEUGLYASNCYS
6   METARGTHRMETGLYTYRMETPROTHRGLU
7   METGLULEUILEGLULEUSERGLNGLNILE
8   ASNMETASNLEUGLYGLYHISVALASPPHE
9   ASPASPPHEVALGLULEUMETGLYPROLYS
10   LEULEUALAGLUTHRALAASPMETILEGLY
11   VALLYSGLULEUARGASPALAPHEARGGLU
12   PHEASPTHRASNGLYASPGLYGLUILESER
13   THRSERGLULEUARGGLUALAMETARGLYS
14   LEULEUGLYHISGLNVALGLYHISARGASP
15   ILEGLUGLUILEILEARGASPVALASPLEU
16   ASNGLYASPGLYARGVALASPPHEGLUGLU
17   PHEVALARGMETMETSERARG

Samples:

sample_1: CaBP1, [U-98% 13C; U-98% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15623 16862 17532
PDB 2LAN 2LAP 3OX5 3OX6
DBJ BAE24603
EMBL CAC43037 CAD20347
GB AAF25782 AAF25784 AAF25787 AAH30201 ABM86439
REF NP_001028847 NP_001028848 NP_001028849 NP_001297641 NP_001297644
SP O88751 Q9NZU7