BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15192

Title: NMR Solution Structure, Stability, and Interaction of the Recombinant Bovine Fibrinogen alphaC-Domain Fragment   PubMed: 17590019

Authors: Burton, Robert; Tsurupa, Galina; Roy, Hantgan; Nico, Tjandra; Leonid, Medved

Citation: Burton, Robert; Tsurupa, Galina; Hantgan, Roy; Tjandra, Nico; Medved, Leonid. "NMR solution structure, stability, and interaction of the recombinant bovine fibrinogen alphaC-domain fragment."  Biochemistry 46, 8550-8560 (2007).

Assembly members:
subunit 1, polymer, 79 residues, 8975.755 Da.

Natural source:   Common Name: not available   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
subunit 1: MIDNEKVTSGHTTTTRRSCS KVITKTVTNADGRTETTKEV VKSEDGSDCGDADFDWHHTF PSRGNLDDFFHRDKDDFFT

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts74
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 79 residues - 8975.755 Da.

1   METILEASPASNGLULYSVALTHRSERGLY
2   HISTHRTHRTHRTHRARGARGSERCYSSER
3   LYSVALILETHRLYSTHRVALTHRASNALA
4   ASPGLYARGTHRGLUTHRTHRLYSGLUVAL
5   VALLYSSERGLUASPGLYSERASPCYSGLY
6   ASPALAASPPHEASPTRPHISHISTHRPHE
7   PROSERARGGLYASNLEUASPASPPHEPHE
8   HISARGASPLYSASPASPPHEPHETHR

Samples:

sample_1: entity_1, [U-99% 13C; U-99% 15N], 150 uM; KPO4 10 mM; NaCl 150 mM; D20 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 282 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAC67562 AAI02565 AAI42073
REF NP_001028798 XP_005217494
SP P02672
TPG DAA20930