BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15185

Title: backbone and side chain methly order parameters and correlation times for calmodulin in complex with the eNOS peptide   PubMed: 17637663

Authors: Frederick, Kendra; Marlow, Michael; Valentine, Kathleen; Wand, Joshua

Citation: Frederick, Kendra; Marlow, Michael; Valentine, Kathleen; Wand, Joshua. "Conformational entropy in molecular recognition by proteins"  Nature 448, 325-329 (2007).

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
eNOSp, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
eNOSp: RKKTFKEVANAVKISASLMG

Data sets:
Data typeCount
order parameters195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2eNOSp2

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, eNOSp 20 residues - Formula weight is not available

derived from the CaM binding region of the human endothelial nitric oxide synthase

1   ARGLYSLYSTHRPHELYSGLUVALALAASN
2   ALAVALLYSILESERALASERLEUMETGLY

Samples:

sample_1: calmodulin, [U-100% 15N], 1 mM; eNOSp 1.2 ± 0.1 mM; potassium chloride 100 mM; imidazole 20 mM; CaCl2 6 mM; sodium azide 3 mM; H2O 95%; D2O 5%

sample_2: calmodulin, [U-100% 13C; U-100% 15N; 55% 2H], 1 mM; eNOSp 1.2 ± 0.1 mM; potassium chloride 100 mM; imidazole 20 mM; CaCl2 6 mM; sodium azide 3 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15184 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310 18028 19586
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAA05652 BAD15356 BAD97356 BAF85617 BAG37648
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA09493 CAA53950 CAI10801
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA30494 AAA30667 AAA30669 AAA36364 AAA36365
PIR JC1305 MCON
PRF 0409298A 0608335A 2011304A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_000594 NP_001003158 NP_001076202 NP_001123373 NP_001153581
SP O02367 O16305 O96081 P02594 P05932 P29473 P29474 P70313 Q62600 Q9TUX8
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA30266