BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15162

Title: Backbone Chemical Shift Assignments of Cholera Toxin Enzymatic Domain (1-167)   PubMed: 18272180

Authors: Ampapathi, Ravi; Lou, In Hye; Creath, Andrea; Blanke, Steven; Legge, Glen

Citation: Ampapathi, Ravi; Creath, Andrea; Lou, Dianne; Craft, John; Blanke, Steven; Legge, Glen. "Order-disorder-order transitions mediate the activation of cholera toxin"  J. Mol. Biol. 377, 748-60 (2008).

Assembly members:
CTA1-T2, polymer, 167 residues, 18891.8 Da.

Natural source:   Common Name: Vibrio comma   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CTA1-T2: NDDKLYRADSRPPDEIKQSG GLMPRGQSDYFDRGTQMNIN LYDHARGTQTGFVRHDDGYV STSISLRSAHLVGQTILSGH STYYIYVIATAPNMFNVNDV LGAYSPHPDEQDVSALGGIP YSQIVGWYRVHFGVLDEQLH RNRGYRDRYYSNLDIAPAAD GYGLAGF

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CTA1-T21

Entities:

Entity 1, CTA1-T2 167 residues - 18891.8 Da.

This is a truncated version(1-167) of Full length CTA1 (1-192), which increases the stability of the construct in solution.

1   ASNASPASPLYSLEUTYRARGALAASPSER
2   ARGPROPROASPGLUILELYSGLNSERGLY
3   GLYLEUMETPROARGGLYGLNSERASPTYR
4   PHEASPARGGLYTHRGLNMETASNILEASN
5   LEUTYRASPHISALAARGGLYTHRGLNTHR
6   GLYPHEVALARGHISASPASPGLYTYRVAL
7   SERTHRSERILESERLEUARGSERALAHIS
8   LEUVALGLYGLNTHRILELEUSERGLYHIS
9   SERTHRTYRTYRILETYRVALILEALATHR
10   ALAPROASNMETPHEASNVALASNASPVAL
11   LEUGLYALATYRSERPROHISPROASPGLU
12   GLNASPVALSERALALEUGLYGLYILEPRO
13   TYRSERGLNILEVALGLYTRPTYRARGVAL
14   HISPHEGLYVALLEUASPGLUGLNLEUHIS
15   ARGASNARGGLYTYRARGASPARGTYRTYR
16   SERASNLEUASPILEALAPROALAALAASP
17   GLYTYRGLYLEUALAGLYPHE

Samples:

sample_1: CTA1-T2 0.2 mM; TRIS, [U-99% 2H], 20 mM; sodium chloride 200 mM; D2O, [U-99% 2H], 6 % v/v; sodium azide 0.05 % w/v; EDTA 1 mM

sample_conditions_1: pH: 7.2; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY v3.110, Goddard - data analysis, peak picking

NMRPipe vScript Version 2002.044.17.08, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw vScript Version 2002.044.17.08, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

GB VC1457 AAA27514 AAD51359 AAF94614 AAL09681 AAL60525
SWS 301555
PDB 1S5B 1S5C 1S5D 1S5E 1S5F 1XTC 2A5D 2A5F 2A5G
DBJ BAA06288 BAA06290 BAG06738 BAG66065 BAI50777
EMBL CAA24995 CAA41590 CAA41592 CAE11218 CRZ40571
PRF 1001196A
REF NP_231100 WP_001881225 WP_001888508 WP_001911232 WP_005514365
SP P01555