BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15145

Title: 1H, Chemical Shift Assignments for neurotensin in HFIP:H20 (80:20), TFE:D20(80:20), and DPC micelles   PubMed: 17441729

Authors: Monti, Jean-Pierre; Coutant, Jerome; Curmi, Patrick

Citation: Coutant, Jerome; Curmi, Patrick; Toma, Flavio; Monti, Jean-Pierre. "NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition"  Biochemistry 46, 5656-5663 (2007).

Assembly members:
neurotensin, polymer, 13 residues, 129.114 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
neurotensin: XLYENKPRRPYIL

Data typeCount
1H chemical shifts326

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1neurotensin1

Entities:

Entity 1, neurotensin 13 residues - 129.114 Da.

1   PCALEUTYRGLUASNLYSPROARGARGPRO
2   TYRILELEU

Samples:

sample_1: neurotensin 5 mM; HFIP 80%; D2O, [U-2H], 20%

sample_2: neurotensin 5 mM; TFE 80%; D2O, [U-2H], 20%

sample_3: neurotensin 5 mM; DPC mM

sample_conditions_1: pressure: 1 atm; temperature: 293 K

sample_conditions_2: pH: 6.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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