BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15142

Title: Solution structure of dynein light chain 2A

Authors: Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.

Citation: Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.. "Solution structure of dynein light chain 2A"  . ., .-..

Assembly members:
Dynein_light_chain_2A,_cytoplasmic, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Dynein_light_chain_2A,_cytoplasmic: GPLGSMAEVEETLKRLQSQK GVQGIIVVNTEGIPIKSTMD NPTTTQYASLMHSFILKARS TVRDIDPQNDLTFLRIRSKK NEIMVAPDKDYFLIVIQNPT E

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts90
1H chemical shifts649

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain A1
2chain B1

Entities:

Entity 1, chain A 101 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETALAGLUVALGLU
2   GLUTHRLEULYSARGLEUGLNSERGLNLYS
3   GLYVALGLNGLYILEILEVALVALASNTHR
4   GLUGLYILEPROILELYSSERTHRMETASP
5   ASNPROTHRTHRTHRGLNTYRALASERLEU
6   METHISSERPHEILELEULYSALAARGSER
7   THRVALARGASPILEASPPROGLNASNASP
8   LEUTHRPHELEUARGILEARGSERLYSLYS
9   ASNGLUILEMETVALALAPROASPLYSASP
10   TYRPHELEUILEVALILEGLNASNPROTHR
11   GLU

Samples:

sample_1: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; H2O 95%; D2O 5%

sample_2: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; D2O 100%

sample_conditions_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_2isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

NMRPipe v2.2, Delaglio - data analysis

PIPP v4.3.2, Garrett - data analysis

X-PLOR v3.1f, Brunger - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG57523
GB AAF29126 AAF86646 AAH02481 AAK18712 AAK95342
REF NP_001177109 NP_001268656 NP_001268657 NP_001268658 NP_054902
SP Q9NP97