BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15141

Title: Chemical shift assignment of TC-1   PubMed: 17905836

Authors: Choy, Wing-Yiu

Citation: Gall, Chris; Xu, Hanyu; Brickenden, Anne; Ai, Xuanjun; Choy, Wing-Yiu. "The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity"  Biochemistry 16, 2510-2518 (2007).

Assembly members:
Thyroid_Cancer_1_(TC-1)_protein, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Thyroid_Cancer_1_(TC-1)_protein: MKAKRSHQAIIMSTSLRVSP SIHGYHFDTASRKKAVGNIF ENTDQESLERLFRNSGDKKA EERAKIIFAIDQDVEEKTRA LMALKKRTKDKLFQFLKLRK YSIKVH

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts75
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TC-11

Entities:

Entity 1, TC-1 106 residues - Formula weight is not available

1   METLYSALALYSARGSERHISGLNALAILE
2   ILEMETSERTHRSERLEUARGVALSERPRO
3   SERILEHISGLYTYRHISPHEASPTHRALA
4   SERARGLYSLYSALAVALGLYASNILEPHE
5   GLUASNTHRASPGLNGLUSERLEUGLUARG
6   LEUPHEARGASNSERGLYASPLYSLYSALA
7   GLUGLUARGALALYSILEILEPHEALAILE
8   ASPGLNASPVALGLUGLULYSTHRARGALA
9   LEUMETALALEULYSLYSARGTHRLYSASP
10   LYSLEUPHEGLNPHELEULYSLEUARGLYS
11   TYRSERILELYSVALHIS

Samples:

Thyroid_Cancer_1: Thyroid Cancer 1 (TC-1) protein, [U-99% 13C; U-99% 15N], 0.1 mM; DSS5 – 10 mM; sodium acetate 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBThyroid_Cancer_1isotropicsample_conditions_1
3D CBCA(CO)NHThyroid_Cancer_1isotropicsample_conditions_1
3D HNCOThyroid_Cancer_1isotropicsample_conditions_1
3D C(CO)NHThyroid_Cancer_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

GB AAF78961 AAH20623 AAH21672 ACE87044 ACE87727
REF NP_001180920 NP_064515 XP_002819077 XP_003734646 XP_003823354
SP Q9NR00