BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15125

Title: N.N.   PubMed: 18815416

Authors: Lin, Y.; Padmanabhan, B.; Yokoyama, S.; Guntert, P.

Citation: Lin, Yi-Jan; Umehara, Takashi; Inoue, Makoto; Saito, Kohei; Kigawa, Takanori; Jang, Moon-Kyoo; Ozato, Keiko; Yokoyama, Shigeyuki; Padmanabhan, Balasundaram; Guntert, Peter. "Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4"  Protein Sci. 17, 2174-2179 (2008).

Assembly members:
Brd4-ET domain, polymer, 90 residues, 10328.770 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli

Entity Sequences (FASTA):
Brd4-ET domain: GSSGSSGESEEEDKCKPMSY EEKRQLSLDINKLPGEKLGR VVHIIQSREPSLKNSNPDEI EIDFETLKPSTLRELERYVT SCLRKKRKPQ

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts87
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Brd4-ET domain1

Entities:

Entity 1, Brd4-ET domain 90 residues - 10328.770 Da.

1   GLYSERSERGLYSERSERGLYGLUSERGLU
2   GLUGLUASPLYSCYSLYSPROMETSERTYR
3   GLUGLULYSARGGLNLEUSERLEUASPILE
4   ASNLYSLEUPROGLYGLULYSLEUGLYARG
5   VALVALHISILEILEGLNSERARGGLUPRO
6   SERLEULYSASNSERASNPROASPGLUILE
7   GLUILEASPPHEGLUTHRLEULYSPROSER
8   THRLEUARGGLULEUGLUARGTYRVALTHR
9   SERCYSLEUARGLYSLYSARGLYSPROGLN

Samples:

sample_1: Brd4-ET domain, [U-100% 13C; U-100% 15N], 1.05 mM; sodium chloride 100 mM; TRIS, [U-2H], 20 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED NOESYsample_1isotropicsample_conditions_1
3D 13C-SEPARATED NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.2.2, P.GUNTERT ET AL. - refinement, structure solution

OPALP v1.4, R.KORADI ET AL. - refinement

NMRView, B.A.JOHNSON - data analysis

Kujira, N.KOBAYASHI - data analysis

NMR spectrometers:

  • BRUKER DRX 600 MHz
  • BRUKER AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE27987 BAE72981
EMBL CAA66186 CAA72780
GB AAC27978 AAG02191 AAH35266 AAI38835 AAI38836
REF NP_001094373 NP_001191701 NP_001273559 NP_055114 NP_065254
SP O60885 Q9ESU6
TPG DAA28223