BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15123

Title: SeV Ntail(443-501)   PubMed: 17459940

Authors: Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence

Citation: Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence. "Interaction of the C-terminal domains of sendai virus N and P proteins: comparison of polymerase-nucleocapsid interactions within the paramyxovirus family"  J. Virol. 81, 6807-6816 (2007).

Assembly members:
SeV_nucleoprotein_Ntail, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Respirovirus   Taxonomy ID: 186938   Superkingdom: Viruses   Kingdom: not available   Genus/species: Respirovirus not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SeV_nucleoprotein_Ntail: GESGERWARQVSGGHFVTLH GAERLEEETNDEDVSDIERR IAMRLAERRQEDSATHGDE

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts70
1H chemical shifts357

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ntail1

Entities:

Entity 1, Ntail 59 residues - Formula weight is not available

1   GLYGLUSERGLYGLUARGTRPALAARGGLN
2   VALSERGLYGLYHISPHEVALTHRLEUHIS
3   GLYALAGLUARGLEUGLUGLUGLUTHRASN
4   ASPGLUASPVALSERASPILEGLUARGARG
5   ILEALAMETARGLEUALAGLUARGARGGLN
6   GLUASPSERALATHRHISGLYASPGLU

Samples:

13C_15N: SeV nucleoprotein Ntail, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 500 mM; potassium phosphate 50 mM

sample_condition_1: ionic strength: 0.5 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC13C_15Nisotropicsample_condition_1
3D CBCA(CO)NH13C_15Nisotropicsample_condition_1
3D HCCH-TOCSY13C_15Nisotropicsample_condition_1
3D C(CO)NH13C_15Nisotropicsample_condition_1
3D HNCACB13C_15Nisotropicsample_condition_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian Avance 600 MHz
  • Varian Avance 800 MHz

Related Database Links:

DBJ BAD74219 BAD74225 BAF73479 BAF73485 BAH59027
EMBL CAA24945 CAA35092 CAA35715
GB AAB06278 AAB06284 AAB06290
SP P04857 P04858 Q07097