BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15117

Title: Backbone H, C, N Chemical Shifts for Influenza A NS1 (1-73) Protein Bound to dsRNA   PubMed: 17475623

Authors: Yin, Cuifeng; Montelione, Gaetano

Citation: Yin, Cuifeng; Khan, Javed; Swapna, Gurla; Ertekin, Asli; Krug, Robert; Tong, Liang; Montelione, Gaetano. "Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses."  J. Biol. Chem. 282, 20584-20592 (2007).

Assembly members:
NS1A, polymer, 73 residues, 8297.416 Da.
stranded_RNA_1, polymer, 16 residues, Formula weight is not available
stranded_RNA_2, polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: influenza A virus A/Udorn/72   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenzavirus A Influenza A virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NS1A: MDSNTVSSFQVDCFLWHVRK QVVDQELGDAPFLDRLRRDQ KSLRGRGSTLGLNIEAATHV GKQIVEKILKEES
stranded_RNA_1: CCAUCCUCUACAGGCG
stranded_RNA_2: CCAUCCUCUACAGGCG

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts87
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS1A_chain 11
2NS1A_chain 21
3dsRNA2
4dsRNA3

Entities:

Entity 1, NS1A_chain 1 73 residues - 8297.416 Da.

1   METASPSERASNTHRVALSERSERPHEGLN
2   VALASPCYSPHELEUTRPHISVALARGLYS
3   GLNVALVALASPGLNGLULEUGLYASPALA
4   PROPHELEUASPARGLEUARGARGASPGLN
5   LYSSERLEUARGGLYARGGLYSERTHRLEU
6   GLYLEUASNILEGLUALAALATHRHISVAL
7   GLYLYSGLNILEVALGLULYSILELEULYS
8   GLUGLUSER

Entity 2, dsRNA 16 residues - Formula weight is not available

1   CCAUCCUCUA
2   CAGGCG

Entity 3, dsRNA 16 residues - Formula weight is not available

1   CCAUCCUCUA
2   CAGGCG

Samples:

NS1A_(1-73): NS1A, [U-100% 13C; U-100% 15N], 0.1 mM; ammonium acetate 50 mM; ds-RNA 0.2 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNS1A_(1-73)isotropicsample_conditions_1
3D HNCONS1A_(1-73)isotropicsample_conditions_1
3D HNCANS1A_(1-73)isotropicsample_conditions_1
3D HNCACBNS1A_(1-73)isotropicsample_conditions_1
3D HN(CO)CACBNS1A_(1-73)isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AutoAssign, Zimmerman, Moseley, Kulikowski, Montelione - data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4317
PDB
DBJ BAA01428
EMBL CAA24288 CAC09426
GB AAA43086 AAA43515 AAA43688 AAC36136 AAC36138
PRF 2005320B 2005320D
SP P03494 P03495 P69277 P69278 Q1PUD3