BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15115

Title: Solution structure of antimicrobial peptide Arenicin-2 in water   PubMed: 17585874

Authors: Nadezhdin, Kirill; Shenkarev, Zakhar

Citation: Ovchinnikovaa, Tatiana; Shenkareva, Zakhar; Nadezhdina, Kirill; Balandina, Sergey; Zhmaka, Maxim; Kudelinaa, Irina; Finkinaa, Ekaterina; Kokryakovb, Vladimir; Arseniev, Alexander. "Recombinant expression, synthesis, purification, and solution structure of arenicin"  Biochem. Biophys. Res. Commun. 360, 156-162 (2007).

Assembly members:
antimicrobial_peptide, polymer, 21 residues, 2780.392 Da.

Natural source:   Common Name: lugworm   Taxonomy ID: 6344   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Arenicola marina

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
antimicrobial_peptide: RWCVYAYVRIRGVLVRYRRC W

Data sets:
Data typeCount
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1arenicin-21

Entities:

Entity 1, arenicin-2 21 residues - 2780.392 Da.

SS-bond Cys3-Cys20

1   ARGTRPCYSVALTYRALATYRVALARGILE
2   ARGGLYVALLEUVALARGTYRARGARGCYS
3   TRP

Samples:

sample_1: arenicin-2 0.6 mM

sample_conditions_1: ionic strength: 0 M; pH: 3.3; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis, processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

VNMR, Varian - collection, data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian Unity 600 MHz

Related Database Links:

BMRB 17430
PDB
GB AAV65143
SP Q5SC59