BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15102

Title: IKs producing slow voltage-gated potassium channel subunit beta, KCNE1   PubMed: 17892302

Authors: Tian, Changlin; Kim, Hak jun; Sanders, Charles

Citation: Tian, Changlin; Vanoye, Carlos; Kang, Congbao; Welch, Richard; Kim, Hak; George, Alfred; Sanders, Charles. "Preparation, Functional Characterization, and NMR Studies of Human KCNE1, a Voltage-Gated Potassium Channel Accessory Subunit Associated with Deafness and Long QT Syndrome(,)"  Biochemistry 46, 11459-11472 (2007).

Assembly members:
KCNE1, polymer, 129 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
KCNE1: MILSNTTAVTPFLTKLWQET VQQGGNMSGLARRSPRSGDG KLEALYVLMVLGFFGFFTLG IMLSYIRSKKLEHSNDPFNV YIESDAWQEKDKAYVQARVL ESYKSCYVVENHLAIEQPNT HLPETKPSP

Data sets:
Data typeCount
13C chemical shifts336
15N chemical shifts112
1H chemical shifts499

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KCNE11

Entities:

Entity 1, KCNE1 129 residues - Formula weight is not available

1   METILELEUSERASNTHRTHRALAVALTHR
2   PROPHELEUTHRLYSLEUTRPGLNGLUTHR
3   VALGLNGLNGLYGLYASNMETSERGLYLEU
4   ALAARGARGSERPROARGSERGLYASPGLY
5   LYSLEUGLUALALEUTYRVALLEUMETVAL
6   LEUGLYPHEPHEGLYPHEPHETHRLEUGLY
7   ILEMETLEUSERTYRILEARGSERLYSLYS
8   LEUGLUHISSERASNASPPROPHEASNVAL
9   TYRILEGLUSERASPALATRPGLNGLULYS
10   ASPLYSALATYRVALGLNALAARGVALLEU
11   GLUSERTYRLYSSERCYSTYRVALVALGLU
12   ASNHISLEUALAILEGLUGLNPROASNTHR
13   HISLEUPROGLUTHRLYSPROSERPRO

Samples:

sample_1: KCNE1, [U-100% 13C; U-100% 15N; 80% 2H], 1 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 6.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe v6.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Bruker AMX 800 MHz
  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAG36319 BAG65181 BAG73568
EMBL CAG46556
GB AAA36129 AAA58418 AAA63905 AAD25096 AAH36452
REF NP_000210 NP_001121140 NP_001121141 NP_001121142 NP_001257331
SP P15382