BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15101

Title: Backbone 1H, 15N, and 13C Resonance Assignment and Secondary Structure Prediction of HP0495 from Helicobacter pylori   PubMed: 17373705

Authors: Seo, Min-Duk

Citation: Seo, Min-Duk; Park, Sung Jean; Kim, Hyun-Jung; Lee, Bong-Jin. "Solution structure of hypothetical protein, HP0495 (Y495_HELPY) from Helicobacter pylori"  Proteins 67, 1189-1192 (2007).

Assembly members:
HP0495, polymer, 86 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HP0495: MPSDSKKPTIIYPCLWDYRV IMTTKDTSTLKELLETYQRP FKLEFKNTSKNAKFYSFNVS MEVSNESERNEIFQKISQLD KVVQTL

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts80
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HP04951

Entities:

Entity 1, HP0495 86 residues - Formula weight is not available

1   METPROSERASPSERLYSLYSPROTHRILE
2   ILETYRPROCYSLEUTRPASPTYRARGVAL
3   ILEMETTHRTHRLYSASPTHRSERTHRLEU
4   LYSGLULEULEUGLUTHRTYRGLNARGPRO
5   PHELYSLEUGLUPHELYSASNTHRSERLYS
6   ASNALALYSPHETYRSERPHEASNVALSER
7   METGLUVALSERASNGLUSERGLUARGASN
8   GLUILEPHEGLNLYSILESERGLNLEUASP
9   LYSVALVALGLNTHRLEU

Samples:

sample_1: HP0495, [U-99% 13C; U-99% 15N], 1.2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15190
PDB
GB AAD07569 ADU82921 AFV41714 AFV43308 AFV44901
REF NP_207292 WP_001138777 WP_001138778
SP O25237