BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15099

Title: Solution structure of CM15 in DPC micelles   PubMed: 17397158

Authors: Respondek, Michal; Madl, Tobias; Goebl, Christoph; Golser, Regina; Zangger, Klaus

Citation: Respondek, Michal; Madl, Tobias; Goebl, Christoph; Golser, Regina; Zangger, Klaus. "Mapping the orientation of helices in micelle-bound peptides by paramagnetic relaxation waves"  J. Am. Chem. Soc. 129, 5228-5234 (2007).

Assembly members:
CM15, polymer, 15 residues, 1776.343 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis   Host organism: not applicable

Entity Sequences (FASTA):
CM15: KWKLFKKIGAVLKVL

Data sets:
Data typeCount
13C chemical shifts26
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CM151

Entities:

Entity 1, CM15 15 residues - 1776.343 Da.

1   LYSTRPLYSLEUPHELYSLYSILEGLYALA
2   VALLEULYSVALLEU

Samples:

sample_1: CM15 1.7 mM; DPC, [U-2H], 100 mM; KPi 50 mM; Sodium azide 0.02%

sample_conditions_1: ionic strength: 0.15 M; pH: 5.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHACANHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCOCAsample_1isotropicsample_conditions_1
3D HCCH-COSY aromsample_1isotropicsample_conditions_1
3D 1H-13C NOESY-HSQCsample_1isotropicsample_conditions_1
3D CCCONHsample_1isotropicsample_conditions_1
3D HNCOCACBsample_1isotropicsample_conditions_1
3D HCCH-COSY RD1sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HBHACACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCCONH TOCSYsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

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