BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15098

Title: SOLUTION STRUCTURES OF THE BRK DOMAINS OF THE HUMAN CHROMO HELICASE DOMAIN 7 AND 8, REVEALS STRUCTURAL SIMILARITY WITH GYF DOMAIN SUGGESTING A ROLE IN PROTEIN INTERACTION

Authors: AB, E.; De Jong, R.; Diercks, T.; Xiaoyun, J.; Daniels, M.; Kaptein, R.; Folkers, G.

Citation: AB, E.; De Jong, R.; Diercks, T.; Xiaoyun, J.; Daniels, M.; Kaptein, R.; Folkers, G.. "Solution Structures of the Brk Domains of the Human Chromo Helicase Domain 7 and 8, Reveals Structural Similarity with Gyf Domain Suggesting a Role in Protein Interaction"  . ., .-..

Assembly members:
CHROMODOMAIN-HELICASE-DNA-BINDING_PROTEIN_8, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):
CHROMODOMAIN-HELICASE-DNA-BINDING_PROTEIN_8: MGSSHHHHHHSSHMLVPRGS LKKLVELEVECMEEPNHLDV DLETRIPVINKVDGTLLVGE DAPRRAELEMWLQGHPEFAV DPRFLAYMEDRRKQK

Data sets:
Data typeCount
13C chemical shifts241
15N chemical shifts53
1H chemical shifts380

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 81

Entities:

Entity 1, CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8 95 residues - Formula weight is not available

1991-2011 His Tag 2012-2085 BRK DOMANI

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERHISMETLEUVALPROARGGLYSER
3   LEULYSLYSLEUVALGLULEUGLUVALGLU
4   CYSMETGLUGLUPROASNHISLEUASPVAL
5   ASPLEUGLUTHRARGILEPROVALILEASN
6   LYSVALASPGLYTHRLEULEUVALGLYGLU
7   ASPALAPROARGARGALAGLULEUGLUMET
8   TRPLEUGLNGLYHISPROGLUPHEALAVAL
9   ASPPROARGPHELEUALATYRMETGLUASP
10   ARGARGLYSGLNLYS

Samples:

sample_1: CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8, [U-13C; U-15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 150 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1.0 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
HNCACBsample_1isotropicsample_conditions_1
CBCACONHsample_1isotropicsample_conditions_1
HBHACONHsample_1isotropicsample_conditions_1
HNCAHAsample_1isotropicsample_conditions_1
CCCONHsample_1isotropicsample_conditions_1
CCH-COSYsample_1isotropicsample_conditions_1
HCCH-TOCSYsample_1isotropicsample_conditions_1
HNH-NOESYsample_1isotropicsample_conditions_1
HCH-NOESYsample_1isotropicsample_conditions_1
CNH-NOESYsample_1isotropicsample_conditions_1
HH-NOESYsample_1isotropicsample_conditions_1

Software:

CNS FOR WATER REFINEMENT vREFINEMENT, BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,RICE,SIMONSON,WARREN - refinement

SPARKY, T Goddard - structure solution

NMR spectrometers:

  • Bruker DMX 700 MHz

Related Database Links:

PDB 2CKA 2DL6