BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15092

Title: 1H, 13C, 15N chemical shift assignments for the human E2 ubiquitin conjugating enzyme Ubc13   PubMed: 17327397

Authors: Mercier, Pascal; Lewis, Michael; Hau, D.; Saltibus, Linda; Xiao, Wei; Spyracopoulos, Leo

Citation: Mercier, Pascal; Lewis, Michael; Hau, D.; Saltibus, Linda; Xiao, Wei; Spyracopoulos, Leo. "Structure, Interactions, and Dynamics of the RING Domain from Human TRAF6"  Protein Sci. 16, 602-614 (2007).

Assembly members:
Ubc13, polymer, 157 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ubc13: GPLGSMAGLPRRIIKETQRL LAEPVPGIKAEPDESNARYF HVVIAGPQDSPFEGGTFKLE LFLPEEYPMAAPKVRFMTKI YHPNVDKLGRICLDILKDKW SPALQIRTVLLSIQALLSAP NPDDPLANDVAEQWKTNEAQ AIETARAWTRLYAMNNI

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts423
15N chemical shifts124
1H chemical shifts721

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubc131

Entities:

Entity 1, Ubc13 157 residues - Formula weight is not available

Residues 1-5 represent a cloning artifact from an affinity tag

1   GLYPROLEUGLYSERMETALAGLYLEUPRO
2   ARGARGILEILELYSGLUTHRGLNARGLEU
3   LEUALAGLUPROVALPROGLYILELYSALA
4   GLUPROASPGLUSERASNALAARGTYRPHE
5   HISVALVALILEALAGLYPROGLNASPSER
6   PROPHEGLUGLYGLYTHRPHELYSLEUGLU
7   LEUPHELEUPROGLUGLUTYRPROMETALA
8   ALAPROLYSVALARGPHEMETTHRLYSILE
9   TYRHISPROASNVALASPLYSLEUGLYARG
10   ILECYSLEUASPILELEULYSASPLYSTRP
11   SERPROALALEUGLNILEARGTHRVALLEU
12   LEUSERILEGLNALALEULEUSERALAPRO
13   ASNPROASPASPPROLEUALAASNASPVAL
14   ALAGLUGLNTRPLYSTHRASNGLUALAGLN
15   ALAILEGLUTHRALAARGALATRPTHRARG
16   LEUTYRALAMETASNASNILE

Samples:

sample_1: Ubc13, [U-13C; U-15N], 0.5 mM; DSS, none, 1 mM; DTT, none, 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - processing

SPARKY, T Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB 1J7D 2C2V 3HCT 3HCU 3VON 3W31 4DHI 4DHJ 4DHZ 4IP3 4NR3 4NRG 4NRI 4ONM 4ONN 4ORH 4TKP 4WHV 5AIT 5AIU
DBJ BAA11675 BAB20414 BAB23941 BAB24239 BAE01994
EMBL CAA71001 CAH65129 CAH92264 CAJ82488 CAK04885
GB AAH00396 AAH03365 AAH34898 AAH44461 AAH53141
REF NP_001012828 NP_001069726 NP_001127530 NP_001156530 NP_001180523
SP P61088 P61089 Q0P5K3 Q4R4I1 Q5R7J6
TPG DAA30062