BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15091

Title: Lipid Free Mouse Apolipoprotein-AI(mAI216)NMR Backbone Assignment   PubMed: 19636841

Authors: Yang, Yunhuang; Wang, Jianjun

Citation: Yang, Yunhuang; Hoyt, David; Wang, Jianjun. "A complete NMR spectral assignment of the lipid-free mouse apolipoprotein A-I (apoAI) C-terminal truncation mutant, apoAI(1-216)"  Biomol. NMR Assignments 1, 109-111 (2007).

Assembly members:
apoprotein_AI, polymer, 240 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apoprotein_AI: DEPQSQWDKVKDFANVYVDA VKDSGRDYVSQFESSSLGQQ LNLNLLENWDTLGSTVSQLQ ERLGPLTRDFWDNLEKETDW VRQEMNKDLEEVKQKVQPYL DEFQKKWKEDVELYRQKASP QGAELQESARQKLQELQGRL SPVAEEFRDRMRTHVDSLRT QLAPHSEQMRESLAQRLAEL KSNPTLNEYHSSAKSHLKSL GEKARPALEDLRHSLMPQLE SLKTKAQSEIDKASENLNAQ

Data sets:
Data typeCount
13C chemical shifts641
15N chemical shifts194
1H chemical shifts1045

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mApoAI2161

Entities:

Entity 1, mApoAI216 240 residues - Formula weight is not available

1   ASPGLUPROGLNSERGLNTRPASPLYSVAL
2   LYSASPPHEALAASNVALTYRVALASPALA
3   VALLYSASPSERGLYARGASPTYRVALSER
4   GLNPHEGLUSERSERSERLEUGLYGLNGLN
5   LEUASNLEUASNLEULEUGLUASNTRPASP
6   THRLEUGLYSERTHRVALSERGLNLEUGLN
7   GLUARGLEUGLYPROLEUTHRARGASPPHE
8   TRPASPASNLEUGLULYSGLUTHRASPTRP
9   VALARGGLNGLUMETASNLYSASPLEUGLU
10   GLUVALLYSGLNLYSVALGLNPROTYRLEU
11   ASPGLUPHEGLNLYSLYSTRPLYSGLUASP
12   VALGLULEUTYRARGGLNLYSALASERPRO
13   GLNGLYALAGLULEUGLNGLUSERALAARG
14   GLNLYSLEUGLNGLULEUGLNGLYARGLEU
15   SERPROVALALAGLUGLUPHEARGASPARG
16   METARGTHRHISVALASPSERLEUARGTHR
17   GLNLEUALAPROHISSERGLUGLNMETARG
18   GLUSERLEUALAGLNARGLEUALAGLULEU
19   LYSSERASNPROTHRLEUASNGLUTYRHIS
20   SERSERALALYSSERHISLEULYSSERLEU
21   GLYGLULYSALAARGPROALALEUGLUASP
22   LEUARGHISSERLEUMETPROGLNLEUGLU
23   SERLEULYSTHRLYSALAGLNSERGLUILE
24   ASPLYSALASERGLUASNLEUASNALAGLN

Samples:

triple_labeled_mAI216: apoprotein AI, [U-13C; U-15N; U-2H], 1.5 mM; D2O, [U-99% 2H], 5%; DSS 0.1 mM; H2O 95%; sodium phosphate 25 mM; EDTA 10 mM; sodium chloride 25 mM; sodium azide 1 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCtriple_labeled_mAI216isotropicsample_conditions_1
3D HNCAtriple_labeled_mAI216isotropicsample_conditions_1
3D HN(CO)CAtriple_labeled_mAI216isotropicsample_conditions_1
3D HN(CA)CBtriple_labeled_mAI216isotropicsample_conditions_1
3D HN(COCA)CBtriple_labeled_mAI216isotropicsample_conditions_1
3D 1H-15N NOESYtriple_labeled_mAI216isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian Unity 900 MHz

Related Database Links:

BMRB 17717
PDB