BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15087

Title: SOLUTION STRUCTURE OF THE PABC DOMAIN FROM TRITICUM AEVESTIUM POLY(A)-BINDING PROTEIN   PubMed: 17358048

Authors: Siddiqui, Nadeem

Citation: Siddiqui, Nadeem; Osborne, Michael; Gallie, Daniel; Gehring, Kalle. "Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants."  Biochemistry 46, 4221-4231 (2007).

Assembly members:
Poly(A)-binding_protein, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: Wheat   Taxonomy ID: 4565   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Triticum aestivum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Poly(A)-binding_protein: GPLGSPIGALASALANSPPE TQRMMLGENLYPLVDQLEHD QAAKVTGMLLEMDQTEVLHL LESPDALKAKVAEAMEVLRS AQQHT

Data sets:
Data typeCount
15N chemical shifts78
1H chemical shifts393

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PABC1

Entities:

Entity 1, PABC 85 residues - Formula weight is not available

This is the C-terminal segment from wheat PABP.

1   GLYPROLEUGLYSERPROILEGLYALALEU
2   ALASERALALEUALAASNSERPROPROGLU
3   THRGLNARGMETMETLEUGLYGLUASNLEU
4   TYRPROLEUVALASPGLNLEUGLUHISASP
5   GLNALAALALYSVALTHRGLYMETLEULEU
6   GLUMETASPGLNTHRGLUVALLEUHISLEU
7   LEUGLUSERPROASPALALEULYSALALYS
8   VALALAGLUALAMETGLUVALLEUARGSER
9   ALAGLNGLNHISTHR

Samples:

sample_1: PABP; Sodium phosphate, Sodium Chloride, Sodium Azide, [U-15N], 1 – 2 mM; phosphate buffer 50 mM; NaCl 150 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: PABP; Sodium phosphate, Sodium Chloride, Sodium Azide, [U-100% 13C; U-100% 15N], 1 – 2 mM; phosphate buffer 50 mM; NaCl 150 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_3: PABP; Sodium phosphate, Sodium Chloride, Sodium Azide, none, 1 – 2 mM; phosphate buffer 50 mM; NaCl 150 mM; NaN3 1 mM; H2O 90%; D2O 10%

sample_4: Poly(A)-binding protein, [U-100% 13C; U-100% 15N], 1 – 2 mM; phosphate buffer 50 mM; NaCl 150 mM; NaN3 1 mM; D2O 100%

sample_conditions_1: ionic strength: 200 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_3isotropicsample_conditions_1
2D TOCSYsample_3isotropicsample_conditions_1
2D NOESYsample_4isotropicsample_conditions_1
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
HNCACBsample_2isotropicsample_conditions_1
CBCACONHsample_2isotropicsample_conditions_1

Software:

CNS v1.1, A Brunger, P Adams, M Clore, P Gros, M Nilges and R Read - refinement

NMR spectrometers:

  • Varian UNITY 800 MHz

Related Database Links:

PDB
DBJ BAJ96406
GB AAB38974 EMS55649 EMT14398