BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15082

Title: HN,CA,CB Chemical shift assignments for apo-Rat intestinal fatty acid binding protein, Clofibric acid-Rat intestinal fatty acid binding protein complex, Fenofibric acid-Rat intestinal fatty acid binding protein complex and Tolfenamic acid-Rat intestinal fatty acid binding protein complex.   PubMed: 17462580

Authors: Velkov, Tony; Scanlon, Martin; Porter, Christopher

Citation: Velkov, Tony; Horne, James; Languerre, Aisha; Jones, Eric; Scanlon, Martin; Porter, Christopher. "Examination of the role of intestinal fatty acid-binding protein in drug absorption using a parallel artificial membrane permeability assay."  Chem. Biol. 14, 453-465 (2007).

Assembly members:
Rat_intestinal_fatty_acid_binding_protein, polymer, 131 residues, 14993.0 Da.

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Rat_intestinal_fatty_acid_binding_protein: AFDGTWKVDRNENYEKFMEK MGINVVKRKLGAHDNLKLTI TQEGNKFTVKESSNFRNIDV VFELGVDFAYSLADGTELTG TWTMEGNKLVGKFKRVDNGK ELIAVREISGNELIQTYTYE GVEAKRIFKKE

Data typeCount
13C chemical shifts248
15N chemical shifts129
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1single polypeptide chain1

Entities:

Entity 1, single polypeptide chain 131 residues - 14993.0 Da.

1   ALAPHEASPGLYTHRTRPLYSVALASPARG
2   ASNGLUASNTYRGLULYSPHEMETGLULYS
3   METGLYILEASNVALVALLYSARGLYSLEU
4   GLYALAHISASPASNLEULYSLEUTHRILE
5   THRGLNGLUGLYASNLYSPHETHRVALLYS
6   GLUSERSERASNPHEARGASNILEASPVAL
7   VALPHEGLULEUGLYVALASPPHEALATYR
8   SERLEUALAASPGLYTHRGLULEUTHRGLY
9   THRTRPTHRMETGLUGLYASNLYSLEUVAL
10   GLYLYSPHELYSARGVALASPASNGLYLYS
11   GLULEUILEALAVALARGGLUILESERGLY
12   ASNGLULEUILEGLNTHRTYRTHRTYRGLU
13   GLYVALGLUALALYSARGILEPHELYSLYS
14   GLU

Samples:

apo-Rat_FABP2: Rat intestinal fatty acid binding protein, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 90%; D2O 10%

sample_cond: ionic strength: 0.05 M; pH: 5.5; temperature: 295.15 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHapo-Rat_FABP2isotropicsample_cond

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAA41133 AAA41138 AAA41141 EDL82110
PRF 1202232A 1202232A:PDB=1IFC
REF NP_037200
SP P02693