BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15081

Title: U2 snRNA stem I from human   PubMed: 17242306

Authors: Sashital, Dipali; Venditti, Vincenzo; Angers, Cortney; Cornilescu, Gabriel; Butcher, Samuel

Citation: Sashital, Dipali; Venditti, Vincenzo; Angers, Cortney; Cornilescu, Gabriel; Butcher, Samuel. "Structure and thermodynamics of a conserved U2 snRNA domain from yeast and human"  RNA 13, 328-338 (2007).

Assembly members:
Human_U2_stem_I, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Human_U2_stem_I: XCUCXXCCXUUUXGCUAAXA

Data sets:
Data typeCount
13C chemical shifts68
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human U2 Stem I1

Entities:

Entity 1, Human U2 Stem I 20 residues - Formula weight is not available

1   PSUCUCOMGOMGCCPSUU
2   UUOMGGCUAAOMGA

Samples:

sample_1: Human U2 stem I 0.5 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: Human U2 stem I 0.5 mM; sodium chloride 50 mM; D2O 99.99%

sample_3: Human U2 stem I 0.5 mM; sodium chloride 50 mM; potassium phosphate 10 mM; Pf1 phage 17 w/v; D2O 99.99%

sample_conditions_1: pH: 7.0; temperature: 283 K

sample_conditions_2: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_3anisotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2

Software:

xwinnmr, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker DMX 750 MHz

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