BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15076

Title: Dynein Light Chain LC8 at pH 5.5   PubMed: 17570393

Authors: Benison, Gregory

Citation: Benison, Gregory; Karplus, P.; Barbar, Elisar. "Structure and dynamics of LC8 complexes with KXTQT-motif peptides: swallow and dynein intermediate chain compete for a common site"  J. Mol. Biol. 371, 457-468 (2007).

Assembly members:
LC8, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LC8: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWHCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
13C chemical shifts87
15N chemical shifts87
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC8, chain 11
2LC8, chain 21

Entities:

Entity 1, LC8, chain 1 89 residues - Formula weight is not available

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: LC8, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; sodium citrate 50 mM; glycerol 3%; DSS 1 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15077 15078 15953 16847 17692
PDB 1RHW 2P2T 2PG1 3BRI 3BRL 3DVH 3DVP 3DVT 3E2B 3FM7 3GLW 4QH7 4QH8
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836
SP O96860 Q24117
TPG DAA34424