BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15076

Title: Dynein Light Chain LC8 at pH 5.5   PubMed: 17570393

Authors: Benison, Gregory

Citation: Benison, Gregory; Karplus, P.; Barbar, Elisar. "Structure and dynamics of LC8 complexes with KXTQT-motif peptides: swallow and dynein intermediate chain compete for a common site"  J. Mol. Biol. 371, 457-468 (2007).

Assembly members:
LC8, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LC8: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWHCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
13C chemical shifts87
15N chemical shifts87
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC8, chain 11
2LC8, chain 21

Entities:

Entity 1, LC8, chain 1 89 residues - Formula weight is not available

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: LC8, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; sodium citrate 50 mM; glycerol 3%; DSS 1 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15077 15078 15953 16847 17692
PDB
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836
SP O96860 Q24117
TPG DAA34424