BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15072

Title: OSCP-NT (1-120) in complex with N-terminal (1-25) alpha subunit from F1-ATPase   PubMed: 17355883

Authors: Carbajo, Rodrigo; Neuhaus, David; Kellas, Fiona; Yang, Ji-Chun; Runswick, Michael; Montgomery, Martin; Walker, John

Citation: Carbajo, Rodrigo; Kellas, Fiona; Yang, Ji-Chun; Runswick, Michael; Montgomery, Martin; Walker, John; Neuhaus, David. "How the N-terminal Domain of the OSCP Subunit of Bovine F1Fo-ATP Synthase Interacts with the N-terminal Region of an Alpha Subunit"  J. Mol. Biol. 368, 310-318 (2007).

Assembly members:
oscp-nt, polymer, 120 residues, Formula weight is not available
alpha-nt, polymer, 25 residues, Formula weight is not available

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
oscp-nt: FAKLVRPPVQIYGIEGRYAT ALYSAASKQNKLEQVEKELL RVGQILKEPKMAASLLNPYV KRSVKVKSLSDMTAKEKFSP LTSNLINLLAENGRLTNTPA VISAFSTMMSVHRGEVPCTV
alpha-nt: QKTGTAEVSSILEERILGAD TSVDL

Data sets:
Data typeCount
13C chemical shifts222
15N chemical shifts100
1H chemical shifts738

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OSCP N-terminal 1-1201
2alpha N-terminal 1-252

Entities:

Entity 1, OSCP N-terminal 1-120 120 residues - Formula weight is not available

1   PHEALALYSLEUVALARGPROPROVALGLN
2   ILETYRGLYILEGLUGLYARGTYRALATHR
3   ALALEUTYRSERALAALASERLYSGLNASN
4   LYSLEUGLUGLNVALGLULYSGLULEULEU
5   ARGVALGLYGLNILELEULYSGLUPROLYS
6   METALAALASERLEULEUASNPROTYRVAL
7   LYSARGSERVALLYSVALLYSSERLEUSER
8   ASPMETTHRALALYSGLULYSPHESERPRO
9   LEUTHRSERASNLEUILEASNLEULEUALA
10   GLUASNGLYARGLEUTHRASNTHRPROALA
11   VALILESERALAPHESERTHRMETMETSER
12   VALHISARGGLYGLUVALPROCYSTHRVAL

Entity 2, alpha N-terminal 1-25 25 residues - Formula weight is not available

1   GLNLYSTHRGLYTHRALAGLUVALSERSER
2   ILELEUGLUGLUARGILELEUGLYALAASP
3   THRSERVALASPLEU

Samples:

sample_1: oscp-nt, [U-99% 13C; U-99% 15N], 0.5 mM; alpha-nt 1.5 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

X-PLOR, Brunger - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAA30676 AAI02205 ELR58089 AAB59266 AAI16060 ACD02421 EFB21996 EGW04262
PRF 1002210A
REF NP_776669 XP_005674722 XP_005893414 XP_005956973 XP_005956974 NP_001172071 NP_001268629 NP_001274083 NP_777109 XP_001506958
SP P13621 P19483 P80021
TPG DAA33707 DAA15776
DBJ BAK09231
EMBL CAA45865