BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15071

Title: NMR Assignments of the apo Corynebacterium diphtheria Heme Oxygenase   PubMed: 19636825

Authors: Hom, Kellie

Citation: Hom, Kellie; Furci, Lena; Deshmukh, Rahul; Wilks, Angela. "NMR assignments of cd-HO, a 24 kDa heme oxygenase from Corynebacterium diphtheria"  Biomol. NMR Assignments 1, 55-56 (2007).

Assembly members:
cdHO, polymer, 215 residues, 24138 Da.

Natural source:   Common Name: Corynebacterium diphtheria   Taxonomy ID: 1717   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cdHO: MTTATAGLAVELKQSTAQAH EKAEHSTFMSDLLKGRLGVA EFTRLQEQAWLFYTALEQAV DAVRASGFAESLLDPALNRA EVLARDLDKLNGSSEWRSRI TASPAVIDYVNRLEEIRDNV DGPALVAHHYVRYLGDLSGG QVIARMMQRHYGVDPEALGF YHFEGIAKLKVYKDEYREKL NNLELSDEQREHLLKEATDA FVFNHQVFADLGKGL

Data sets:
Data typeCount
13C chemical shifts505
15N chemical shifts180
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cdHO1

Entities:

Entity 1, cdHO 215 residues - 24138 Da.

1   METTHRTHRALATHRALAGLYLEUALAVAL
2   GLULEULYSGLNSERTHRALAGLNALAHIS
3   GLULYSALAGLUHISSERTHRPHEMETSER
4   ASPLEULEULYSGLYARGLEUGLYVALALA
5   GLUPHETHRARGLEUGLNGLUGLNALATRP
6   LEUPHETYRTHRALALEUGLUGLNALAVAL
7   ASPALAVALARGALASERGLYPHEALAGLU
8   SERLEULEUASPPROALALEUASNARGALA
9   GLUVALLEUALAARGASPLEUASPLYSLEU
10   ASNGLYSERSERGLUTRPARGSERARGILE
11   THRALASERPROALAVALILEASPTYRVAL
12   ASNARGLEUGLUGLUILEARGASPASNVAL
13   ASPGLYPROALALEUVALALAHISHISTYR
14   VALARGTYRLEUGLYASPLEUSERGLYGLY
15   GLNVALILEALAARGMETMETGLNARGHIS
16   TYRGLYVALASPPROGLUALALEUGLYPHE
17   TYRHISPHEGLUGLYILEALALYSLEULYS
18   VALTYRLYSASPGLUTYRARGGLULYSLEU
19   ASNASNLEUGLULEUSERASPGLUGLNARG
20   GLUHISLEULEULYSGLUALATHRASPALA
21   PHEVALPHEASNHISGLNVALPHEALAASP
22   LEUGLYLYSGLYLEU

Samples:

sample_1: cdHO, [U-13C; U-15N; U-2H], 1 – 2 mM; H2O 95%; D2O 5%; DSS 1 mM; potassium phosphate 50 mM; potassium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.11, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15070 15073
PDB 1IW0 1IW1 1V8X 1WNV 1WNW 1WNX 1WZD 1WZF 1WZG 2Z68 3I8R 3MOO 4GOH 4GPC 4GPF 4GPH
DBJ BAA76407
EMBL CAE50198 CKH06070
GB AAC44832 AEX42338 AEX44660 AEX46855 AEX67845
REF WP_003852259 WP_010935240 WP_014302152 WP_014303664 WP_014307115
SP P71119