BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15065

Title: 2-PGA-bound WT chicken Triosephosphate Isomerase (TIM)   PubMed: 17336327

Authors: Kempf, James; Jung, Ju-yeon; Ragain, Christina; Sampson, Nicole; Loria, Pat

Citation: Kempf, James; Jung, Ju-yeon; Ragain, Christina; Sampson, Nicole; Loria, J.. "Dynamic Requirements for a Functional Protein Hinge"  J. Mol. Biol. 368, 131-149 (2007).

Assembly members:
triosephosphate_isomerase, polymer, 248 residues, 26620 Da.
PGA, non-polymer, 156.031 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
triosephosphate_isomerase: MAPRKFFVGGNWKMNGDKKS LGELIHTLNGAKLSADTEVV CGAPSIYLDFARQKLDAKIG VAAQNCYKVPKGAFTGEISP AMIKDIGAAWVILGHSERRH VFGESDELIGQKVAHALAEG LGVIACIGEKLDEREAGITE KVVFEQTKAIADNVKDWSKV VLAYEPVWAIGTGKTATPQQ AQEVHEKLRGWLKSHVSDAV AQSTRIIYGGSVTGGNCKEL ASQHDVDGFLVGGASLKPEF VDIINAKH

Data sets:
Data typeCount
13C chemical shifts449
15N chemical shifts218
1H chemical shifts218
heteronuclear NOE values432
T1 relaxation values432
T2 relaxation values431

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WT cTIM chain 11
2WT cTIM chain 21
32-PGA2

Entities:

Entity 1, WT cTIM chain 1 248 residues - 26620 Da.

26.6kDa monomer of 53.1 kDa homodimer reported

1   METALAPROARGLYSPHEPHEVALGLYGLY
2   ASNTRPLYSMETASNGLYASPLYSLYSSER
3   LEUGLYGLULEUILEHISTHRLEUASNGLY
4   ALALYSLEUSERALAASPTHRGLUVALVAL
5   CYSGLYALAPROSERILETYRLEUASPPHE
6   ALAARGGLNLYSLEUASPALALYSILEGLY
7   VALALAALAGLNASNCYSTYRLYSVALPRO
8   LYSGLYALAPHETHRGLYGLUILESERPRO
9   ALAMETILELYSASPILEGLYALAALATRP
10   VALILELEUGLYHISSERGLUARGARGHIS
11   VALPHEGLYGLUSERASPGLULEUILEGLY
12   GLNLYSVALALAHISALALEUALAGLUGLY
13   LEUGLYVALILEALACYSILEGLYGLULYS
14   LEUASPGLUARGGLUALAGLYILETHRGLU
15   LYSVALVALPHEGLUGLNTHRLYSALAILE
16   ALAASPASNVALLYSASPTRPSERLYSVAL
17   VALLEUALATYRGLUPROVALTRPALAILE
18   GLYTHRGLYLYSTHRALATHRPROGLNGLN
19   ALAGLNGLUVALHISGLULYSLEUARGGLY
20   TRPLEULYSSERHISVALSERASPALAVAL
21   ALAGLNSERTHRARGILEILETYRGLYGLY
22   SERVALTHRGLYGLYASNCYSLYSGLULEU
23   ALASERGLNHISASPVALASPGLYPHELEU
24   VALGLYGLYALASERLEULYSPROGLUPHE
25   VALASPILEILEASNALALYSHIS

Entity 2, 2-PGA - C2 H5 O6 P - 156.031 Da.

1   PGA

Samples:

2H_15N_sample: triosephosphate isomerase, [U-99% 15N; U-99%2H], 0.9 mM; D2O 7.5%; sodium azide 0.02%; sodium chloride 10 mM; MES, [U-2H], 10 mM; 2-phosphoglycolate 2 mM

2H_13C_15N_sample: triosephosphate isomerase, [U-99% 13C; U-99% 15N; 70% 2H], 0.9 mM; D2O 7.5%; sodium azide 0.02%; sodium chloride 10 mM; MES, [U-2H], 10 mM; 2-phosphoglycolate 2 mM

standard_conditions: ionic strength: . M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCA2H_13C_15N_sampleisotropicstandard_conditions
3D HN(CO)CA2H_13C_15N_sampleisotropicstandard_conditions
3D HNCACB2H_13C_15N_sampleisotropicstandard_conditions
3D HN(CO)CACB2H_13C_15N_sampleisotropicstandard_conditions
3D HN(CA)CO2H_13C_15N_sampleisotropicstandard_conditions
3D HNCO2H_13C_15N_sampleisotropicstandard_conditions
1H-15N ssNOE2H_15N_sampleisotropicstandard_conditions
1H-15N T12H_15N_sampleisotropicstandard_conditions
1H-15N T22H_15N_sampleisotropicstandard_conditions

Software:

SPARKY, Goddard - data analysis

PISTACHIO, Eghbalnia - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15064 15066 15067
PDB
EMBL CAE45562 CAE45563 CAE45564
GB AAA49094 AAA49095 ACH45461 ACH45462 ACH45463
REF NP_001232159 NP_990782 XP_005016819 XP_005235418 XP_005434378
SP P00940