BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15058

Title: 1H assignment of En-6   PubMed: 17663000

Authors: Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt

Citation: Pedrini, Bill; Placzek, William; Koculi, Eda; Alimenti, Claudio; LaTerza, Antonietta; Luporini, Pierangelo; Wuthrich, Kurt. "Cold-adaptation in sea-water-borne signal proteins: Sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii"  J. Mol. Biol. 372, 277-286 (2007).

Assembly members:
En-6, polymer, 63 residues, Formula weight is not available

Natural source:   Common Name: Euplotes nobilii   Taxonomy ID: 184062   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Euplotes nobilii

Experimental source:   Production method: purified from the natural source   Host organism: Euplotes nobilii

Entity Sequences (FASTA):
En-6: TDPEEHFDPNTNCDYTNSQD AWDYCTNYIVNSSCGEICCN DCFDETGTGACRAQAFGNSC LNW

Data sets:
Data typeCount
1H chemical shifts344

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1En-6 monomer1

Entities:

Entity 1, En-6 monomer 63 residues - Formula weight is not available

1   THRASPPROGLUGLUHISPHEASPPROASN
2   THRASNCYSASPTYRTHRASNSERGLNASP
3   ALATRPASPTYRCYSTHRASNTYRILEVAL
4   ASNSERSERCYSGLYGLUILECYSCYSASN
5   ASPCYSPHEASPGLUTHRGLYTHRGLYALA
6   CYSARGALAGLNALAPHEGLYASNSERCYS
7   LEUASNTRP

Samples:

sample_1: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM

sample_2: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM

sample_3: En-6 1 ± 0.2 mM; sodium phosphate 20 ± 0.5 mM; DSS 1 ± 0.1 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
1D 1Hsample_3isotropicsample_conditions_1

Software:

CARA v1.5.3, Keller, R - chemical shift assignment

XWINNMR, Bruker Biospin - collection

ATNOS-CANDID v1.2, Guntert, Mumenthaler and Wuthrich, Herrmann, Guntert and Wuthrich - peak assignment, peak picking, structure solution

OPALp, Koradi, Billeter and Guntert - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - peak assignment, peak picking, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ABK15649