BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15052

Title: HPRP-173-195-D178N SOLUTION STRUCTURE   PubMed: 18563793

Authors: Saviano, G.; Tancredi, T.

Citation: Ronga, Luisa; Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Benedetti, Ettore; Ragone, Raffaele; Rossi, Filomena. "Structural characterization of a neurotoxic threonine-rich peptide corresponding to the human prion protein alpha2-helical 180-195 segment, and comparison with full-length alpha2-helix-derived peptides"  J. Pept. Sci. 14, 1096-1102 (2008).

Assembly members:
HPRP-173-195-D178N, polymer, 23 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HPRP-173-195-D178N: NNFVHNCVNITIKQHTVTTT TKG

Data sets:
Data typeCount
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mutated prion protein fragment1

Entities:

Entity 1, mutated prion protein fragment 23 residues - Formula weight is not available

1   ASNASNPHEVALHISASNCYSVALASNILE
2   THRILELYSGLNHISTHRVALTHRTHRTHR
3   THRLYSGLY

Samples:

sample_1: HPRP-173-195-D178N1 – 2 mM; TRIFLUOROETHANOL-D2, [U-2H], 100%

sample_conditions_1: pH: 5.0; pressure: 1.0 atm; temperature: 300.0 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
15N-HSQCsample_1isotropicsample_conditions_1

Software:

DYANA, P.GUNTERT - refinement

NMRView, B Johnson, One Moon Scientific - structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 15676
PDB 2IV6 2K1D 3HEQ 3HJX