BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15036

Title: SOLUTION STRUCTURE OF HUMAN BETA-MICROSEMINOPROTEIN   PubMed: 16930619

Authors: Ghasriani, H.; Teilum, K.; Johnsson, Y.; Fernlund, P.; Drakenberg, T.

Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structure of human and porcine beta-microseminoprotein."  J. Mol. Biol. 362, 502-515 (2006).

Assembly members:
hMSP, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hMSP: GGGSCYFIPNEGVPGDSTRK CMDLKGNKHPINSEWQTDNC ETCTCYETEISCCTLVSTPV GYDKDNCQRIFKKEDCKYIV VEKKDPKKTCSVSEWII

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts98
1H chemical shifts591

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hMSP1

Entities:

Entity 1, hMSP 97 residues - Formula weight is not available

1   GLYGLYGLYSERCYSTYRPHEILEPROASN
2   GLUGLYVALPROGLYASPSERTHRARGLYS
3   CYSMETASPLEULYSGLYASNLYSHISPRO
4   ILEASNSERGLUTRPGLNTHRASPASNCYS
5   GLUTHRCYSTHRCYSTYRGLUTHRGLUILE
6   SERCYSCYSTHRLEUVALSERTHRPROVAL
7   GLYTYRASPLYSASPASNCYSGLNARGILE
8   PHELYSLYSGLUASPCYSLYSTYRILEVAL
9   VALGLULYSLYSASPPROLYSLYSTHRCYS
10   SERVALSERGLUTRPILEILE

Samples:

sample_1: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; H20 90%; D20 10%

sample_2: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; D20 100%

sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 310.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, CLORE - refinement

NMR spectrometers:

  • VARIAN UNITYINOVA 600 MHz

Related Database Links:

BMRB 15037
PDB
EMBL CAA41002 CAB39325 CAG33296
GB AAA36635 AAA59871 AAB29732 AAB37355 AAH05257
PRF 1209281A
REF NP_002434
SP P08118