BMRB Entry 15036

Name: SOLUTION STRUCTURE OF HUMAN BETA-MICROSEMINOPROTEIN
Published: 2007-01-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15036

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF HUMAN BETA-MICROSEMINOPROTEIN

Deposition date:

2006-11-17

Original release date:

2007-01-10

Authors:

Ghasriani, H.; Teilum, K.; Johnsson, Y.; Fernlund, P.; Drakenberg, T.

Citation:

Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structure of human and porcine beta-microseminoprotein." J. Mol. Biol. 362, 502-515 (2006).
PubMed: 16930619

Assembly members:

Assembly members:
hMSP, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30 Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hMSP: GGGSCYFIPNEGVPGDSTRK CMDLKGNKHPINSEWQTDNC ETCTCYETEISCCTLVSTPV GYDKDNCQRIFKKEDCKYIV VEKKDPKKTCSVSEWII

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	354
15N chemical shifts	98
1H chemical shifts	591

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hMSP	1

Entities:

Entity 1, hMSP 97 residues - Formula weight is not available

1

GLY

SER

CYS

TYR

PHE

ILE

PRO

ASN

2

GLU

GLY

VAL

PRO

GLY

ASP

SER

THR

ARG

LYS

3

CYS

MET

ASP

LEU

LYS

GLY

ASN

LYS

HIS

PRO

4

ILE

ASN

SER

GLU

TRP

GLN

THR

ASP

ASN

CYS

5

GLU

THR

CYS

THR

CYS

TYR

GLU

THR

GLU

ILE

6

SER

CYS

THR

LEU

VAL

SER

THR

PRO

VAL

7

GLY

TYR

ASP

LYS

ASP

ASN

CYS

GLN

ARG

ILE

8

PHE

LYS

GLU

ASP

CYS

LYS

TYR

ILE

VAL

9

VAL

GLU

LYS

ASP

PRO

LYS

THR

CYS

10

SER

VAL

SER

GLU

TRP

ILE

Samples:

sample_1: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; H20 90%; D20 10%

sample_2: entity, [U-95% 13C; U-95% 15N], 3.4 – 4.2 mM; D20 100%

sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 310.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Software:

X-PLOR NIH, CLORE - refinement

NMR spectrometers:

VARIAN UNITYINOVA 600 MHz

BMRB	15037
PDB	2IZ3 3IX0
EMBL	CAA41002 CAB39325 CAG33296
GB	AAA36635 AAA59871 AAB29732 AAB37355 AAH05257
PRF	1209281A
REF	NP_002434
SP	P08118
AlphaFold	P08118