BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15025

Title: Backbone 1H Chemical Shift Assignments for peptide sMTM7 from subunit a of proton V-ATPase   PubMed: 17573038

Authors: Duarte, Afonso; Hemminga, Marcus; de Jong, Edwin; van Mierlo, Carlo; Wechselberger, Rainer

Citation: Duarte, Afonso; de Jong, Edwin; Wechselberger, Rainer; van Mierlo, Carlo; Hemminga, Marcus. "Segment TM7 from the cytoplasmic hemi-channel from VO-H+-V-ATPase includes a flexible region that has a potential role in proton translocation."  Biochim Biophys Acta. 1768, 2263-2270 (2007).

Assembly members:
sMTM7, polymer, 25 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
sMTM7: EFCLNCVSHTASYLRLWALS LAHAQ

Data sets:
Data typeCount
1H chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sMTM71

Entities:

Entity 1, sMTM7 25 residues - Formula weight is not available

This peptide mimicks the seventh transmembrane segmetne of subunit a from the yeast proton B-ATPase

1   GLUPHECYSLEUASNCYSVALSERHISTHR
2   ALASERTYRLEUARGLEUTRPALALEUSER
3   LEUALAHISALAGLN

Samples:

sample_1: sMTM7 2 mM; DMSO, [U-100% 2H], 100%

sample_conditions_1: temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, T Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 900 MHz

Related Database Links:

BMRB 11056
PDB 2JTW 2NVJ 2RPW 3J9T 3J9U 3J9V
DBJ GAA26581
EMBL CAA61776 CAA99494 CAG58212 CAG78894 CAG79347
GB AAA35211 ABN67012 AHY77549 AJP41779 AJT71201
REF NP_014913 XP_001385041 XP_001387315 XP_001483440 XP_001485135
SP P32563
TPG DAA11036