BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15020

Title: Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide   PubMed: 17229736

Authors: Volkman, B.; Peterson, F.; Deng, Q.

Citation: Peterson, F.; Deng, Q.; Zettl, M.; Prehoda, K.; Lim, W.; Way, M.; Volkman, B.. "Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP"  J. Biol. Chem. 282, 8446-8453 (2007).

Assembly members:
WIP-EVH1_polypeptide, polymer, 169 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
WIP-EVH1_polypeptide: GSESRFYFHPISDLPPPEPY VQTTKSYPSKLARNESRGGL VPRGSGGSLFSFLGKKCVTM SSAVVQLYAADRNCMWSKKC SGVACLVKDNPQRSYFLRIF DIKDGKLLWEQELYNNFVYN SPRGYFHTFAGDTCQVALNF ANEEEAKKFRKAVTDLLGRR QRKSEKRRD

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts578
15N chemical shifts139
1H chemical shifts918

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WIP-EVH1 polypeptide1

Entities:

Entity 1, WIP-EVH1 polypeptide 169 residues - Formula weight is not available

1   GLYSERGLUSERARGPHETYRPHEHISPRO
2   ILESERASPLEUPROPROPROGLUPROTYR
3   VALGLNTHRTHRLYSSERTYRPROSERLYS
4   LEUALAARGASNGLUSERARGGLYGLYLEU
5   VALPROARGGLYSERGLYGLYSERLEUPHE
6   SERPHELEUGLYLYSLYSCYSVALTHRMET
7   SERSERALAVALVALGLNLEUTYRALAALA
8   ASPARGASNCYSMETTRPSERLYSLYSCYS
9   SERGLYVALALACYSLEUVALLYSASPASN
10   PROGLNARGSERTYRPHELEUARGILEPHE
11   ASPILELYSASPGLYLYSLEULEUTRPGLU
12   GLNGLULEUTYRASNASNPHEVALTYRASN
13   SERPROARGGLYTYRPHEHISTHRPHEALA
14   GLYASPTHRCYSGLNVALALALEUASNPHE
15   ALAASNGLUGLUGLUALALYSLYSPHEARG
16   LYSALAVALTHRASPLEULEUGLYARGARG
17   GLNARGLYSSERGLULYSARGARGASP

Samples:

sample_1: WIP-EVH1 polypeptide, [U-99% 13C; U-99% 15N], 1.0 mM; sodium phosphate, none, 20 mM; Dithiothreitol, none, 1 mM; H2O, none, 90 mM; D2O, none, 10 mM

sample_conditions_1: ionic strength: 0.044 M; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
3D 13C-separated NOESYsample_1isotropicsample_conditions_1
3D 13C-separated NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

xwinnmr v3.5, Bruker - collection

NMRPipe v2004, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

SPSCAN v1.1.0, R.W. Glaser - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB 2IFS
DBJ BAA11082 BAA20128 BAA21534 BAB29159 BAE24270
EMBL CAC69994 CAH89371 CAH91010 CAL26602
GB AAH19951 AAH52955 AAH55045 AAH58642 AAI51608
REF NP_001103835 NP_001128797 NP_001161217 NP_001253726 NP_003932
SP O00401 O08816 Q91YD9 Q95107
TPG DAA30422