BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15012

Title: chemical shift assignment of the protein Atu4866 from Agrobacterium tumefaciens   PubMed: 18042676

Authors: Ai, Xuanjun; Semesi, Anthony; Yee, Adelinda; Arrowsmith, Cheryl; Li, Shawn; Choy, Wing-Yiu

Citation: Ai, Xuanjun; Semesi, Anthony; Yee, Adelinda; Arrowsmith, Cheryl; Choy, Wing-Yiu; Li, Shawn. "The hypothetical protein Atu4866 from Agrobacterium tumefaciens adopts a streptavidin-like fold"  Protein Sci. 17, 154-158 (2008).

Assembly members:
Atu4866, polymer, 100 residues, Formula weight is not available

Natural source:   Common Name: Agrobacterium tumefaciens   Taxonomy ID: 358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Agrobacterium tumefaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Atu4866: MGSSHHHHHHSSGRENLYFQ GMQHPYVGIWVTADGRIRQE LLPNGRYDEARGNRKSAYQG RYEVRGAHINYWDDTGFTAD GDFVSANELHHGGMTFYREK

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts73
1H chemical shifts458

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Atu48661

Entities:

Entity 1, Atu4866 100 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYMETGLNHISPROTYRVALGLYILETRP
4   VALTHRALAASPGLYARGILEARGGLNGLU
5   LEULEUPROASNGLYARGTYRASPGLUALA
6   ARGGLYASNARGLYSSERALATYRGLNGLY
7   ARGTYRGLUVALARGGLYALAHISILEASN
8   TYRTRPASPASPTHRGLYPHETHRALAASP
9   GLYASPPHEVALSERALAASNGLULEUHIS
10   HISGLYGLYMETTHRPHETYRARGGLULYS

Samples:

sample_1: Atu4866, [U-13C; U-15N], 0.3 – 0.5 mM; sodium acetate mM; NaCl mM; Zn2+ mM; DTT uM; NaN3 mM; inhibitor mixture%; benzamidine mM

sample_conditions_1: ionic strength: 0.45 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2, B.A. Johnson, R.A. Blevins - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAK88581 KEY50003 KJX85412
REF NP_355796 WP_010974236 WP_046033740