BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15009

Title: Backbone and Side Chain 1H, 13C, 15N Chemical Shift Assignments for Sporulation Phosphotransferase F Mutant I90A   PubMed: 17350627

Authors: McLaughlin, Patrick; Bobay, Benjamin; Regel, Erin; Thomspon, Richele; Hoch, James; Cavanagh, John

Citation: McLaughlin, Patrick; Bobay, Benjamin; Regel, Erin; Thomspon, Richele; Hoch, James; Cavanagh, John. "Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition"  FEBS Lett. 581, 1425-1429 (2007).

Assembly members:
Spo0FI90A, polymer, 144 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Spo0FI90A: MMNEKILIVDDQYGIRILLN EVFNKEGYQTFQAANGLQAL DIVTKERPDLVLLDMKIPGM DGIEILKRMKVIDENIRVII MTAYGELDMAQESKELGALT HFAKPFDIDEIRDAVKKYLP LKSNLEHHHHHHLKSNLEHH HHHH

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts523
15N chemical shifts127
1H chemical shifts840

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Spo0FI90A monomer1

Entities:

Entity 1, Spo0FI90A monomer 144 residues - Formula weight is not available

1   METMETASNGLULYSILELEUILEVALASP
2   ASPGLNTYRGLYILEARGILELEULEUASN
3   GLUVALPHEASNLYSGLUGLYTYRGLNTHR
4   PHEGLNALAALAASNGLYLEUGLNALALEU
5   ASPILEVALTHRLYSGLUARGPROASPLEU
6   VALLEULEUASPMETLYSILEPROGLYMET
7   ASPGLYILEGLUILELEULYSARGMETLYS
8   VALILEASPGLUASNILEARGVALILEILE
9   METTHRALATYRGLYGLULEUASPMETALA
10   GLNGLUSERLYSGLULEUGLYALALEUTHR
11   HISPHEALALYSPROPHEASPILEASPGLU
12   ILEARGASPALAVALLYSLYSTYRLEUPRO
13   LEULYSSERASNLEUGLUHISHISHISHIS
14   HISHISLEULYSSERASNLEUGLUHISHIS
15   HISHISHISHIS

Samples:

sample_1: Spo0FI90A, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM; TRIS, none, 25 mM; potassium chloride, none, 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRView v5, B Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15008 15010 15011
PDB 1F51 1FSP 1NAT 1PEY 1PUX 1SRR 2FSP 2FTK 2JVI 2JVJ 2JVK 3Q15
DBJ BAI87374 BAM55796 BAM59808 GAK81027
EMBL CAA27217 CAA89872 CAB15730 CCU60811 CEI59521
GB AAA16802 AAA22787 ADM39703 ADP34224 ADV94523
PRF 1306301A
REF NP_391594 WP_003221952 WP_003227621 WP_010328945 WP_013390680
SP P06628