BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15007

Title: Structural Studies on Plasmodium vivax Merozoite Surface Protein-1   PubMed: 17343930

Authors: Babon, Jeffrey; Morgan, William; Geoff, Kelly; John, Eccleston; James, Feeney; Anthony, Holder

Citation: Babon, Jeffrey; Morgan, William; Kelly, Geoff; Eccleston, John; Feeney, James; Holder, Anthony. "Structural studies on Plasmodium vivax merozoite surface protein-1."  Mol. Biochem. Parasitol. 153, 31-40 (2007).

Assembly members:
msp-1, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: PLASMODIUM VIVAX   Taxonomy ID: 5855   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium vivax

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
msp-1: TMSSEHTCIDTNVPDNAACY RYLDGTEEWRCLLTFKEEGG KCVPASNVTCKDNNGGCAPE AECKMTDSNKIVCKCTKEGS EPLFEGVFCS

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts87
1H chemical shifts487

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1msp-11

Entities:

Entity 1, msp-1 90 residues - Formula weight is not available

1   THRMETSERSERGLUHISTHRCYSILEASP
2   THRASNVALPROASPASNALAALACYSTYR
3   ARGTYRLEUASPGLYTHRGLUGLUTRPARG
4   CYSLEULEUTHRPHELYSGLUGLUGLYGLY
5   LYSCYSVALPROALASERASNVALTHRCYS
6   LYSASPASNASNGLYGLYCYSALAPROGLU
7   ALAGLUCYSLYSMETTHRASPSERASNLYS
8   ILEVALCYSLYSCYSTHRLYSGLUGLYSER
9   GLUPROLEUPHEGLUGLYVALPHECYSSER

Samples:

sample_1: msp-1, [U-100% 13C; U-100% 15N], 0.5 – 1.0 mM; k-phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, CD Schwieters, JJ Kuszewski, N Tjandra and GM Clore - structure solution

NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAJ41307 BAJ41308 BAJ41309 BAJ41310 BAJ41311
EMBL CAC82066 CAC82067 CAC82068 CAC82069 CAC82070
GB AAA29735 AAA63427 AAC37236 AAC37237 AAF91156
REF XP_001614842