BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11595

Title: Solution structure of Zalpha domain of goldfish ZBP-containing protein kinase

Authors: Lee, Ae-Ree; Park, Chin-Ju; Park, Jin-Wan; Kwon, Mun-Young; Choi, Yong-Geun; Kim, Kyeong Kyu; Choi, Byong-Seok; LEE, Joon-Hwa

Citation: Lee, Ae-Ree; Park, Chin-Ju; Park, Jin-Wan; Kwon, Mun-Young; Choi, Yong-Geun; Kim, Kyeong Kyu; Choi, Byong-Seok; Lee, Joon-Hwa. "Solution structure of the Z-DNA binding domain of PKR-like protein kinase from Carassius auratus and quantitative analyses of the intermediate complex during B-Z transition"  Nucleic Acids Res. ., .-. (2016).

Assembly members:
entity, polymer, 64 residues, 7458.576 Da.

Natural source:   Common Name: goldfish   Taxonomy ID: 7957   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Carassius auratus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MSAETQMERKIIDFLRQNGK SIALTIAKEIGLDKSTVNRH LYNLQRSNQVFNSNEKPPVW DLME

Data sets:
Data typeCount
13C chemical shifts169
15N chemical shifts70
1H chemical shifts412

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 64 residues - 7458.576 Da.

1   METSERALAGLUTHRGLNMETGLUARGLYS
2   ILEILEASPPHELEUARGGLNASNGLYLYS
3   SERILEALALEUTHRILEALALYSGLUILE
4   GLYLEUASPLYSSERTHRVALASNARGHIS
5   LEUTYRASNLEUGLNARGSERASNGLNVAL
6   PHEASNSERASNGLULYSPROPROVALTRP
7   ASPLEUMETGLU

Samples:

sample_1: caZapkz, [U-99% 13C; U-99% 15N], 1.0 mM; sodium chloride 100 mM; sodium phosphate 10 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Agilent DD2 700 MHz

Related Database Links: