BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11583

Title: Solution structure of rat P2X4 receptor head domain

Authors: Abe, Yoshito; Igawa, Tatsuhiro; Tsuda, Makoto; Inoue, Kazuhide; Ueda, Tadashi

Citation: Igawa, Tatsuhiro; Abe, Yoshito; Tsuda, Makoto; Inoue, Kazuhide; Ueda, Tadashi. "Solution structure of rat P2X4 receptor head domain involved in inhibitory metal binding."  FEBS Lett. ., .-..

Assembly members:
P2XHD, polymer, 58 residues, 6066.745 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
P2XHD: MQTQSTCPEIPDKTSICNSD ADCTPGSVDTHSSGVATGRC VPFNESVKTCEVAAWCPV

Data sets:
Data typeCount
1H chemical shifts353
13C chemical shifts227
15N chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P2XHD monomer1

Entities:

Entity 1, P2XHD monomer 58 residues - 6066.745 Da.

1   METGLNTHRGLNSERTHRCYSPROGLUILE
2   PROASPLYSTHRSERILECYSASNSERASP
3   ALAASPCYSTHRPROGLYSERVALASPTHR
4   HISSERSERGLYVALALATHRGLYARGCYS
5   VALPROPHEASNGLUSERVALLYSTHRCYS
6   GLUVALALAALATRPCYSPROVAL

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.5 ± 0.2 mM; Urea 50 mM; sodium acetate 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: temperature: 297 K; pH: 5.0; pressure: 1 atm; ionic strength: 0 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert P. - structure solution

TALOS, Cornilescu, Delaglio and Bax - data analysis

Olivia, Hokkaido University - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA61037 CAA62607 CAA63778
GB AAA99777 AAH78792 EDM13668 EDM13669 EDM13670
PRF 2124312A
REF NP_113782
SP P51577