BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11539

Title: Tachyplesin I in water   PubMed: 24389234

Authors: Kushibiki, Takahiro; Kamiya, Masakatsu; Aizawa, Tomoyasu; Kumaki, Yasuhiro; Kikukawa, Takashi; Mizuguchi, Mineyuki; Demura, Makoto; Kawabata, Syun-ichiro; Kawano, Keiichi

Citation: Kushibiki, Takahiro; Kamiya, Masakatsu; Aizawa, Tomoyasu; Kumaki, Yasuhiro; Kikukawa, Takashi; Mizuguchi, Mineyuki; Demura, Makoto; Kawabata, Syun-ichiro; Kawano, Keiichi. "Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide."  Biochim. Biophys. Acta 1844, 527-534 (2014).

Assembly members:
Tachyplesin_I, polymer, 18 residues, Formula weight is not available

Natural source:   Common Name: Tachypleus tridentatus   Taxonomy ID: 6853   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tachypleus tridentatus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Tachyplesin_I: KWCFRVCYRGICYRRCRX

Data sets:
Data typeCount
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TP I1

Entities:

Entity 1, TP I 18 residues - Formula weight is not available

1   LYSTRPCYSPHEARGVALCYSTYRARGGLY
2   ILECYSTYRARGARGCYSARGNH2

Samples:

sample_1: Tachyplesin I 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker DMX 500 MHz
  • JEOL ECA 600 MHz

Related Database Links:

BMRB 1135 11538
PDB
GB AAA63538
SP P14213 P69135 P69136