BMRB Entry 11458

Title:
Solution structure of the N-terminal RNA recognition motif of NonO
Deposition date:
2011-11-29
Original release date:
2012-12-18
Authors:
Nagata, Takashi; Muto, Yutaka; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Makiko; Yokoyama, Shigeyuki
Citation:

Citation: Nagata, Takashi. "Solution structure of the N-terminal RNA recognition motif of NonO"  .

Assembly members:

Assembly members:
entity, polymer, 99 residues, 10909.508 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: not applicable

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts75
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal RNA recognition motif of NonO1

Entities:

Entity 1, N-terminal RNA recognition motif of NonO 99 residues - 10909.508 Da.

1   GLYSERSERGLYSERSERGLYGLYGLULYS
2   THRPHETHRGLNARGSERARGLEUPHEVAL
3   GLYASNLEUPROPROASPILETHRGLUGLU
4   GLUMETARGLYSLEUPHEGLULYSTYRGLY
5   LYSALAGLYGLUVALPHEILEHISLYSASP
6   LYSGLYPHEGLYPHEILEARGLEUGLUTHR
7   ARGTHRLEUALAGLUILEALALYSVALGLU
8   LEUASPASNMETPROLEUARGGLYLYSGLN
9   LEUARGVALARGPHEALACYSHISSERALA
10   SERLEUTHRSERGLYPROSERSERGLY

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.2 mM; TRIS, [U-100% 2H], 20 mM; DTT 1 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB23598 BAB28857 BAC25890 BAE01678 BAE26278
EMBL CAA72157 CAG32298 CAG33042 CAH89438 CAJ18449
GB AAA03427 AAB27887 AAC37578 AAC51852 AAH02364
PIR A54691
REF NP_001012356 NP_001026703 NP_001040019 NP_001124612 NP_001138880
SP Q15233 Q5FVM4 Q5RFL9 Q99K48
TPG DAA12926
AlphaFold Q99K48 Q5FVM4 Q15233 Q5RFL9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks