BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11441

Title: NMR chemical shift of bryum coronatum chitinase   PubMed: 21367878

Authors: Shinya, Shoko; Ohnuma, Takayuki; Taira, Toki; Nishimura, Shigenori; Fukamizo, Tamo

Citation: Taira, Toki; Mahoe, Yoko; Kawamoto, Noriko; Onaga, Shoko; Iwasaki, Hironori; Ohnuma, Takayuki; Fukamizo, Tamo. "Cloning and characterization of a small family 19 chitinase from moss (Bryum coronatum)."  Glycobiology 21, 644-654 (2011).

Assembly members:
Bryum coronatum chitinase, polymer, 205 residues, Formula weight is not available

Natural source:   Common Name: Moss   Taxonomy ID: 216087   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Bryum coronatum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Bryum coronatum chitinase: QSWTSFVTPAVFEGWFPNRN PFYTYDGLVSASNGYPEFGT TGSLDDQKRELAAFLGNINQ ESGGLQFIQEQNPQSDYCDT SSTQYPCAAGKQYYGRGPIQ LSWNYNYGEAGADLGLDLLN NPDLVAQDSTVAWRTALWFW MKRDCHGAITASPPSFSGTI RIINGGLECNQPAGSIGNMQ MENRVTYYTQFCQTLGVDPG TDLRC

Data sets:
Data typeCount
13C chemical shifts578
15N chemical shifts192
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bryum coronatum chitinase1

Entities:

Entity 1, Bryum coronatum chitinase 205 residues - Formula weight is not available

1   GLNSERTRPTHRSERPHEVALTHRPROALA
2   VALPHEGLUGLYTRPPHEPROASNARGASN
3   PROPHETYRTHRTYRASPGLYLEUVALSER
4   ALASERASNGLYTYRPROGLUPHEGLYTHR
5   THRGLYSERLEUASPASPGLNLYSARGGLU
6   LEUALAALAPHELEUGLYASNILEASNGLN
7   GLUSERGLYGLYLEUGLNPHEILEGLNGLU
8   GLNASNPROGLNSERASPTYRCYSASPTHR
9   SERSERTHRGLNTYRPROCYSALAALAGLY
10   LYSGLNTYRTYRGLYARGGLYPROILEGLN
11   LEUSERTRPASNTYRASNTYRGLYGLUALA
12   GLYALAASPLEUGLYLEUASPLEULEUASN
13   ASNPROASPLEUVALALAGLNASPSERTHR
14   VALALATRPARGTHRALALEUTRPPHETRP
15   METLYSARGASPCYSHISGLYALAILETHR
16   ALASERPROPROSERPHESERGLYTHRILE
17   ARGILEILEASNGLYGLYLEUGLUCYSASN
18   GLNPROALAGLYSERILEGLYASNMETGLN
19   METGLUASNARGVALTHRTYRTYRTHRGLN
20   PHECYSGLNTHRLEUGLYVALASPPROGLY
21   THRASPLEUARGCYS

Samples:

sample_1: BcChiA, [U-99% 13C; U-99% 15N], 0.4 mM; sodium acetate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOCAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 11466
PDB
DBJ BAF99002