BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11377

Title: Solution structure of the first C2H2 type zinc finger domain of Zinc finger protein 278

Authors: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the first C2H2 type zinc finger domain of Zinc finger protein 278"  . ., .-..

Assembly members:
zinc finger domain, polymer, 66 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
zinc finger domain: GSSGSSGLREAGILPCGLCG KVFTDANRLRQHEAQHGVTS LQLGYIDLPPPRLGENGLPI SGPSSG

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts53
1H chemical shifts383

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zinc finger domain1
2ZINC ION2

Entities:

Entity 1, zinc finger domain 66 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUARGGLU
2   ALAGLYILELEUPROCYSGLYLEUCYSGLY
3   LYSVALPHETHRASPALAASNARGLEUARG
4   GLNHISGLUALAGLNHISGLYVALTHRSER
5   LEUGLNLEUGLYTYRILEASPLEUPROPRO
6   PROARGLEUGLYGLUASNGLYLEUPROILE
7   SERGLYPROSERSERGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zinc finger domain, [U-13C; U-15N], 0.89 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9820, Kobayashi N. - data analysis

CYANA v2.1, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA90874 BAD92024 BAE38619 BAF84492 BAG10581
EMBL CAB51404 CAG30499 CAK54641 CAK54940
GB AAF01349 AAF32517 AAF32518 AAF99602 AAG09031
REF NP_001100701 NP_001164822 NP_001178126 NP_001240619 NP_001240620
SP Q9HBE1
TPG DAA20504 DAA20505