BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11360

Title: Assigned chemical shifts of the zf-CW domain with H3 trimethyl K4 peptide   PubMed: 20826339

Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation: He, Fahu; Umehara, Takashi; Saito, Kohei; Harada, Takushi; Watanabe, Satoru; Yabuki, Takashi; Kigawa, Takanori; Takahashi, Mari; Kuwasako, Kanako; Tsuda, Kengo; Matsuda, Takayoshi; Aoki, Masaaki; Seki, Eiko; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the zinc finger CW domain as a histone modification reader."  Structure 18, 1127-1139 (2010).

Assembly members:
zf-CW domain, polymer, 69 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
H3 trimethyl K4 peptide, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
zf-CW domain: GSSGSSGEISGFGQCLVWVQ CSFPNCGKWRRLCGNIDPSV LPDNWSCDQNTDVQYNRCDI PEETWTGLE
H3 trimethyl K4 peptide: ARTXQTARKSTGGKAPRKQL

Data sets:
Data typeCount
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-CW domain1
2ZINC ION2
3H3 trimethyl K4 peptide3

Entities:

Entity 1, zf-CW domain 69 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUILESER
2   GLYPHEGLYGLNCYSLEUVALTRPVALGLN
3   CYSSERPHEPROASNCYSGLYLYSTRPARG
4   ARGLEUCYSGLYASNILEASPPROSERVAL
5   LEUPROASPASNTRPSERCYSASPGLNASN
6   THRASPVALGLNTYRASNARGCYSASPILE
7   PROGLUGLUTHRTRPTHRGLYLEUGLU

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Entity 3, H3 trimethyl K4 peptide 20 residues - Formula weight is not available

1   ALAARGTHRM3LGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU

Samples:

sample_1: zf-CW domain mM; H3 trimethyl K4 peptide mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9819, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 11115 11358 11359 11361 11362 11359
PDB
DBJ BAA91424 BAC04028 BAG62414 BAG63489
EMBL CAB66669 CAT02683 CAT02685 CAT02686 CAT02687 CAT02689
GB AAH02725 AIC60275 EAW76538 EAW76539 EAW76540 AAT28135 AAT28136 AAT28137 AAT28138 AAT28139
REF NP_001244937 NP_060454 XP_002803268 XP_003318693 XP_003318694 XP_001194483 XP_002028918 XP_002904927 XP_002927153 XP_003502247
SP Q9H0M4