BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11342

Title: Solution Structure of the RING domain of the human RING-box protein 2

Authors: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the human RING-box protein 2"  . ., .-..

Assembly members:
RING domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
RING domain: GSSGSSGMWSWDVECDTCAI CRVQVMDACLRCQAENKQED CVVVWGECNHSFHNCCMSLW VKQNNRCPLCQQDWVVQRIG K

Data sets:
Data typeCount
13C chemical shifts329
15N chemical shifts91
1H chemical shifts522

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain1
2ZINC ION no.12
3ZINC ION no.22
4ZINC ION no.32

Entities:

Entity 1, RING domain 81 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETTRPSER
2   TRPASPVALGLUCYSASPTHRCYSALAILE
3   CYSARGVALGLNVALMETASPALACYSLEU
4   ARGCYSGLNALAGLUASNLYSGLNGLUASP
5   CYSVALVALVALTRPGLYGLUCYSASNHIS
6   SERPHEHISASNCYSCYSMETSERLEUTRP
7   VALLYSGLNASNASNARGCYSPROLEUCYS
8   GLNGLNASPTRPVALVALGLNARGILEGLY
9   LYS

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE II 900 MHz

Related Database Links:

PDB
DBJ BAB22666 BAE26843 BAG34921 BAJ20342
EMBL CAF94886 CAG32193 CDQ67670 CDQ72674
GB AAD25961 AAD25962 AAD30147 AAD55984 AAH05966
REF NP_001012516 NP_001026478 NP_001069188 NP_001100318 NP_001134211
SP Q9UBF6 Q9WTZ1
TPG DAA33088