BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11279

Title: 2DZI/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Ubiquitin-like Protein 4A (GDX)

Authors: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "2DZI/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Ubiquitin-like Protein 4A (GDX)"  . ., .-..

Assembly members:
ubiquitin-like domain, polymer, 81 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGMQLTVKALQGREC SLQVPEDELVSTLKQLVSEK LNVPVRQQRLLFKGKALADG KRLSDYSIGPNSKLNLVVKP L

Data sets:
Data typeCount
13C chemical shifts347
15N chemical shifts81
1H chemical shifts571

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin-like domain1

Entities:

Entity 1, ubiquitin-like domain 81 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETGLNLEU
2   THRVALLYSALALEUGLNGLYARGGLUCYS
3   SERLEUGLNVALPROGLUASPGLULEUVAL
4   SERTHRLEULYSGLNLEUVALSERGLULYS
5   LEUASNVALPROVALARGGLNGLNARGLEU
6   LEUPHELYSGLYLYSALALEUALAASPGLY
7   LYSARGLEUSERASPTYRSERILEGLYPRO
8   ASNSERLYSLEUASNLEUVALVALLYSPRO
9   LEU

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9748, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG56766
EMBL CAH93235
GB AAA36790 AAA92650 AAH43346 AAH53589 ABZ10495
REF NP_001126901 NP_001164852 NP_001229350 NP_001253630 NP_055050
SP B0KWT6 B1MTV8 B7NZQ9 P11441 Q5R4T1