BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11231

Title: Solution structure of the PKD domain from KIAA 1837 protein

Authors: Qin, X.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PKD domain from KIAA 1837 protein"  . ., .-..

Assembly members:
PKD domain, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PKD domain: GSSGSSGQADAGPDKELTLP VDSTTLDGSKSSDDQKIISY LWEKTQGPDGVQLENANSSV ATVTGLQVGTYVFTLTVKDE RNLQSQSSVNVIVKEESGPS SG

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts98
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PKD domain1

Entities:

Entity 1, PKD domain 102 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNALAASP
2   ALAGLYPROASPLYSGLULEUTHRLEUPRO
3   VALASPSERTHRTHRLEUASPGLYSERLYS
4   SERSERASPASPGLNLYSILEILESERTYR
5   LEUTRPGLULYSTHRGLNGLYPROASPGLY
6   VALGLNLEUGLUASNALAASNSERSERVAL
7   ALATHRVALTHRGLYLEUGLNVALGLYTHR
8   TYRVALPHETHRLEUTHRVALLYSASPGLU
9   ARGASNLEUGLNSERGLNSERSERVALASN
10   VALILEVALLYSGLUGLUSERGLYPROSER
11   SERGLY

Samples:

sample_1: PKD domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB