BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11195

Title: Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11

Authors: Inoue, K.; Suetake, T.; Hayashi, F.; Yokoyama, S.

Citation: Inoue, K.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11"  . ., .-..

Assembly members:
PDZ domain, polymer, 93 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PDZ domain: GSSGSSGVQRCVIIQKDQHG FGFTVSGDRIVLVQSVRPGG AAMKAGVKEGDRIIKVNGTM VTNSSHLEVVKLIKSGAYVA LTLLGSSSGPSSG

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts83
1H chemical shifts588

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 93 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALGLNARG
2   CYSVALILEILEGLNLYSASPGLNHISGLY
3   PHEGLYPHETHRVALSERGLYASPARGILE
4   VALLEUVALGLNSERVALARGPROGLYGLY
5   ALAALAMETLYSALAGLYVALLYSGLUGLY
6   ASPARGILEILELYSVALASNGLYTHRMET
7   VALTHRASNSERSERHISLEUGLUVALVAL
8   LYSLEUILELYSSERGLYALATYRVALALA
9   LEUTHRLEULEUGLYSERSERSERGLYPRO
10   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAH57394 EAW52892 EHH15364 EHH50383
REF NP_937879 XP_001116843 XP_001167782 XP_002810045 XP_003937918