BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11194

Title: Solution structure of the fifth SH3 domain from human KIAA0418 protein

Authors: Inoue, K.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.

Citation: Inoue, K.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fifth SH3 domain from human KIAA0418 protein"  . ., .-..

Assembly members:
SH3 domain, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
SH3 domain: GSSGSSGNLKDVYVSIADYE GDEETAGFQEGVSMEVLERN PNGWWYCQILDGVKPFKGWV PSNYLEKKNSGPSSG

Data sets:
Data typeCount
13C chemical shifts294
15N chemical shifts70
1H chemical shifts455

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 75 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNLEULYS
2   ASPVALTYRVALSERILEALAASPTYRGLU
3   GLYASPGLUGLUTHRALAGLYPHEGLNGLU
4   GLYVALSERMETGLUVALLEUGLUARGASN
5   PROASNGLYTRPTRPTYRCYSGLNILELEU
6   ASPGLYVALLYSPROPHELYSGLYTRPVAL
7   PROSERASNTYRLEUGLULYSLYSASNSER
8   GLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

Delta NMR v4.3.2, JEOL - collection

NMRPipe v20060324, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9807, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • JEOL ECA 800 MHz

Related Database Links:

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