BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11187

Title: Solution structure of the 7th PDZ domain of InaD-like protein

Authors: Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.

Citation: Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the 7th PDZ domain of InaD-like protein"  . ., .-..

Assembly members:
PDZ domain, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PDZ domain: GSSGSSGDAFTDQKIRQRYA DLPGELHIIELEKDKNGLGL SLAGNKDRSRMSIFVVGINP EGPAAADGRMRIGDELLEIN NQILYGRSHQNASAIIKTAP SKVKLVFIRNEDAVNQMASG PSSG

Data sets:
Data typeCount
13C chemical shifts499
15N chemical shifts121
1H chemical shifts818

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 124 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPALAPHE
2   THRASPGLNLYSILEARGGLNARGTYRALA
3   ASPLEUPROGLYGLULEUHISILEILEGLU
4   LEUGLULYSASPLYSASNGLYLEUGLYLEU
5   SERLEUALAGLYASNLYSASPARGSERARG
6   METSERILEPHEVALVALGLYILEASNPRO
7   GLUGLYPROALAALAALAASPGLYARGMET
8   ARGILEGLYASPGLULEULEUGLUILEASN
9   ASNGLNILELEUTYRGLYARGSERHISGLN
10   ASNALASERALAILEILELYSTHRALAPRO
11   SERLYSVALLYSLEUVALPHEILEARGASN
12   GLUASPALAVALASNGLNMETALASERGLY
13   PROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
REF XP_003361134 XP_008592429