BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11172

Title: 13C and 15N chemical shifts of the membrane-reconstituted subunit c-ring of E. coli H+-ATP synthase   PubMed: 20596883

Authors: Todokoro, Yasuto; Kobayashi, Masatoshi; Sato, Takeshi; Kawakami, Toru; Yumen, Ikuko; Aimoto, Saburo; Fujiwara, Toshimichi; Akutsu, Hideo

Citation: Todokoro, Yasuto; Kobayashi, Masatoshi; Sato, Takeshi; Kawakami, Toru; Yumen, Ikuko; Aimoto, Saburo; Fujiwara, Toshimichi; Akutsu, Hideo. "Structural analysis of the membrane-reconstituted subunit c-ring of E. coli H+-ATP synthase by solid-state NMR"  J. Biomol. NMR 48, 1-11 (2010).

Assembly members:
FoF1 ATP synthase subunit c (EFoc), polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FoF1 ATP synthase subunit c (EFoc): MENLNMDLLYMAAAVMMGLA AIGAAIGIGILGGKFLEGAA RQPDLIPLLRTQFFIVMGLV DAIPMIAVGLGLYVMFAVA

Data sets:
Data typeCount
13C chemical shifts127
15N chemical shifts35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FoF1 ATP synthase subunit c (EFoc)1

Entities:

Entity 1, FoF1 ATP synthase subunit c (EFoc) 79 residues - Formula weight is not available

1   METGLUASNLEUASNMETASPLEULEUTYR
2   METALAALAALAVALMETMETGLYLEUALA
3   ALAILEGLYALAALAILEGLYILEGLYILE
4   LEUGLYGLYLYSPHELEUGLUGLYALAALA
5   ARGGLNPROASPLEUILEPROLEULEUARG
6   THRGLNPHEPHEILEVALMETGLYLEUVAL
7   ASPALAILEPROMETILEALAVALGLYLEU
8   GLYLEUTYRVALMETPHEALAVALALA

Samples:

sample_1: EFoc-ring, [U-13C; U-15N], 6 mg; DMPC-d54, 2H-hydrocarbon chain, 21.3 mg; H2O10 – 50%; NaN3 1 mM; Tris-HCl 10 mM

sample_2: EFoc-ring, [3-13C]-Ala24, [4-13C]-Asp61, 6 mg; H2O 100%; DMPC-d54, 2H-hydrocarbon chain, 21.3 mg; NaN3 1 mM; Tris-HCl 10 mM

condition_233K: pH: 8.0; temperature: 233 K

condition_223K: pH: 8.0; temperature: 223 K

condition_193K: pH: 8.0; temperature: 193 K

Experiments:

NameSampleSample stateSample conditions
Intra-residue 2D 13C-13C DARRsample_1solidcondition_233K
Inter-residue 2D CACAsample_1solidcondition_233K
Intra-residue 2D NCACBsample_1solidcondition_233K
Inter-residue 2D N(CO)CACBsample_1solidcondition_233K
Inter-residue 3D N(CO)CAsample_1solidcondition_233K
Intra-residue 3D NCACOsample_1solidcondition_233K
2H-selective 1H-depolarization 13C-NMR(CODSHD)sample_1solidcondition_223K
13C rotational resonancesample_2solidcondition_193K

Software:

FELIX v2007, Felix NMR Inc., San Diego, CA - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian Infinity-plus 600 MHz
  • Varian Infinity-plus 500 MHz

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