BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11058

Title: Chemical shift assignment of PACAP21 bound to phospholipid membranes by magic angle spinning solid-state NMR   PubMed: 17996724

Authors: Komi, Nobuyasu; Okawa, Kayo; Tateishi, Yukihiro; Shirakawa, Masahiro; Fujiwara, Toshimichi; Akutsu, Hideo

Citation: Komi, Nobuyasu; Okawa, Kayo; Tateishi, Yukihiro; Shirakawa, Masahiro; Fujiwara, Toshimichi; Akutsu, Hideo. "Structural analysis of pituitary adenylate cyclase-activating polypeptides bound to phospholipid membranes by magic angle spinning solid-state NMR."  Biochim. Biophys. Acta 1768, 3001-3011 (2007).

Assembly members:
PACAP21, polymer, 21 residues, 2522.9 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PACAP21: HSDGIFTDSYSRYRKQMAVK X

Data sets:
Data typeCount
13C chemical shifts104
15N chemical shifts15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PACAP211

Entities:

Entity 1, PACAP21 21 residues - 2522.9 Da.

1   HISSERASPGLYILEPHETHRASPSERTYR
2   SERARGTYRARGLYSGLNMETALAVALLYS
3   LYS_NH2

Samples:

sample_1: PACAP21, [U-13C; U-15N], 3.8 mg; DPPC, [U-98% 2H], 43.2 uM; DPPG, [U-98% 2H], 10.8 uM

sample_conditions_213K: pressure: 1 atm; temperature: 213 K

sample_conditions_223K: pressure: 1 atm; temperature: 223 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_223K
2D 15N-13Csample_1isotropicsample_conditions_213K
2D 15N-13C DARRsample_1isotropicsample_conditions_213K
2D (CACB)-CA DARRsample_1isotropicsample_conditions_213K

Software:

FELIX v2002, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian Infinity-plus 500 MHz
  • Varian Infinity-plus 600 MHz
  • Varian Infinity-plus 700 MHz

Related Database Links:

BMRB 11059
PDB
DBJ BAA28355 BAC21153 BAC21154 BAC21155 BAC21156
EMBL CAA42962 CAA51705 CAA55684 CAA56564 CAG10213
GB AAA31575 AAA41791 AAB20402 AAB21469 AAB21470
PRF 2107317A
REF NP_001001544 NP_001009776 NP_001040020 NP_001081947 NP_001093203
SP O70176 P0DJ95 P13589 P16613 P18509
TPG DAA15829