BMRB Entry 11016

Title:
Humal Insulin Mutant B31Lys -B32Arg
Deposition date:
2007-12-06
Original release date:
2008-06-27
Authors:
Bocian, Wojciech; Kozerski, Lech
Citation:

Citation: Bocian, Wojciech; Borowicz, Piotr; Mikolajczyk, Jerzy; Sitkowski, Jerzy; Tarnowska, Anna; Bednarek, Elzbieta; Glabski, Tadeusz; Tejchman-Malecka, Bozena; Bogiel, Monika; Kozerski, Lech. "NMR structure of biosynthetic engineered human insulin monomer B31(Lys)-B32(Arg) in water /acetonitrile solution. Comparison with the solution structure of native human insulin monomer."  Biopolymers 89, 820-830 (2008).
PubMed: 18491415

Assembly members:

Assembly members:
Chain A, polymer, 21 residues, 2383.700 Da.
Chain B, polymer, 32 residues, 3721.365 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDBAST-RAT-N-7-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chain A: GIVEQCCTSICSLYQLENYC N
Chain B: FVNQHLCGSHLVEALYLVCG ERGFFYTPKTKR

Data sets:
Data typeCount
1H chemical shifts361

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B2

Entities:

Entity 1, Chain A 21 residues - 2383.700 Da.

1   GLYILEVALGLUGLNCYSCYSTHRSERILE
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, Chain B 32 residues - 3721.365 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERHIS
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRPROLYSTHR
4   LYSARG

Samples:

sample_1: Chain A 2.0 mM; Chain B 2.0 mM; CD3CN 35%; D2O 65%

sample_2: Chain A 2.0 mM; Chain B 2.0 mM; CD3CN 35%; H2O 65%

sample_conditions_1: pH: 3.6; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement, structure solution

NMR spectrometers:

  • Varian Uniform NMR System 500 MHz

Related Database Links:

BMRB 1000 1002 1004 1006 1008 1010 1012 1014 1016 1018 1020 1022 1023 1344 15464 1585 1587 16026 16027 1632 16343 16608 16663 16915 17107 1761 17803 18858 18859 18921 18923 18924 18925 19822 19978 19979 20052 20053 25260 25261 4266 1015 1023 1025 15464 1633 16915 25260 4266 555 557 937
PDB
DBJ BAH59081 BAJ17943 BAM29044
EMBL CAA23424 CAA23475 CAA23828 CAA43403 CAA43405
GB AAA17540 AAA19033 AAA36849 AAA59172 AAA59173 AAA72171 AAA72917 AAC78289
PRF 0601246A 1006230A 550086A 560164B 580107B 0510475A 600165A 640291A
REF NP_000198 NP_001008996 NP_001075804 NP_001103242 NP_001123565
SP P01308 P01311 P01315 P01321 P30406 P01316
PIR INEL
AlphaFold P01315 P01308 P01311 P01321 P30406 P01316